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Forum: Sample Prep / Library Generation 10-12-2020, 10:30 AM
Replies: 20
Views: 6,656
Posted By Rosmano
Oh, just an update: it worked beautifully! :) ...

Oh, just an update: it worked beautifully! :)

I prepared a few new libraries. Could you guys provide feedback and tell me what do you think of these new samples?

https://ibb.co/nbWRn1p...
Forum: Sample Prep / Library Generation 12-01-2019, 05:15 AM
Replies: 20
Views: 6,656
Posted By Rosmano
I did a left side size selection and got the...

I did a left side size selection and got the following band in the Fragment Analyzer. I stored my samples at -20ºC, so now I am going to do the right-side size selection as you describe. I was hoping...
Forum: Sample Prep / Library Generation 12-01-2019, 05:09 AM
Replies: 20
Views: 6,656
Posted By Rosmano
This means you add 1mL of cold ATAC-RSB...

This means you add 1mL of cold ATAC-RSB containing 0.1% Tween-20 to the 50uL to wash the sample
Forum: Sample Prep / Library Generation 08-20-2019, 02:47 AM
Replies: 20
Views: 6,656
Posted By Rosmano
Yup, I got everything working by using solely the...

Yup, I got everything working by using solely the NEBNext master mix. :D

Now, I'm just wondering about performing bead clean-up... I've decided to do a Fragmet analyzer before doing size...
Forum: Sample Prep / Library Generation 08-14-2019, 02:10 AM
Replies: 20
Views: 6,656
Posted By Rosmano
By the way, I am doing the qPCR quantification...

By the way, I am doing the qPCR quantification with Power SYBR Green PCR Master Mix from Thermo Fisher instead of the NEB Next HF Master Mix.

Does anyone know if this might impact the qPCR...
Forum: Sample Prep / Library Generation 08-12-2019, 09:53 AM
Replies: 20
Views: 6,656
Posted By Rosmano
Well, after analyzing my qPCR results again, what...

Well, after analyzing my qPCR results again, what I have to say is that the PCR looks awful but there's something there. Unfortunately, by the end of the 20 cycles I didn't hit a plateau stage and...
Forum: Sample Prep / Library Generation 08-10-2019, 09:15 AM
Replies: 20
Views: 6,656
Posted By Rosmano
What protease inhibitor did you use in your...

What protease inhibitor did you use in your protocol? I adopted the Omni-ATAC protocol but there's no qPCR amplification, even though I have DNA (7.6ng/uL obtained from 50,000 thymocytes).

I don't...
Forum: Sample Prep / Library Generation 04-03-2019, 02:16 AM
Replies: 20
Views: 6,656
Posted By Rosmano
Hey, did you use the new Nextera DNA flex Library...

Hey, did you use the new Nextera DNA flex Library Prep kit?
I've been having similar problems to you but nothing I do seems to work.
Showing results 1 to 8 of 8

 


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