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Forum: Sample Prep / Library Generation 12-01-2019, 08:10 PM
Replies: 20
Views: 6,656
Posted By Meyana
I can't see why there should be any problem in...

I can't see why there should be any problem in doing the upper size selection now, I would just try and see how it sequences.

Hope it works.
Forum: Sample Prep / Library Generation 08-20-2019, 04:53 PM
Replies: 20
Views: 6,656
Posted By Meyana
I always do both upper and lower size selection...

I always do both upper and lower size selection using AMPure XP beads, I don't see a reason not to just do it right away (unless you are unfamiliar with bead cleanup and want to have a way to check...
Forum: Sample Prep / Library Generation 08-14-2019, 02:34 AM
Replies: 20
Views: 6,656
Posted By Meyana
I started out using the PCR mix in the Nextera...

I started out using the PCR mix in the Nextera kit for both PCR and qPCR and got little to no amplification. Switching all steps to NEB (as described in the Omni paper) got everything working...
Forum: Sample Prep / Library Generation 12-13-2018, 04:38 PM
Replies: 20
Views: 6,656
Posted By Meyana
I have seen the same thing repeatedly with FACS...

I have seen the same thing repeatedly with FACS sorted nuclei isolated from frozen tissue, hoping someone has an idea on how to optimize...
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