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Forum: 454 Pyrosequencing 02-02-2015, 01:40 PM
Replies: 11
Views: 8,476
Posted By hoytpr
That is interesting. Maybe we can request some...

That is interesting. Maybe we can request some new oil... that was a problem in the past if I recall. May I ask how you finally decided on 11 minutes and 30 seconds for emPCR extension?

Five...
Forum: 454 Pyrosequencing 10-30-2014, 11:33 AM
Replies: 11
Views: 8,476
Posted By hoytpr
Martin, Thank you for the offer. I visited...

Martin,
Thank you for the offer. I visited your website and it is very nice. Perhaps we can negotiate if no other people are able to help, and Roche cannot help either.

We will remember you and...
Forum: 454 Pyrosequencing 10-28-2014, 07:51 AM
Replies: 11
Views: 8,476
Posted By hoytpr
Thanks Anthony, We also tried to mix those...

Thanks Anthony,
We also tried to mix those chemistries without success, but I am much more concerned about potential software glitches, or that we have somehow just "missed" something. Dr Hong in my...
Forum: 454 Pyrosequencing 10-21-2014, 07:46 AM
Replies: 11
Views: 8,476
Posted By hoytpr
Question Problems since long-read "upgrade". Just us?

So there may not be many of us left, but those who are still using 454-Junior machines... have you had as many problems with the upgrade to long reads as we have had? Before the upgrade, we never had...
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