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Forum: De novo discovery 08-09-2019, 07:58 AM
Replies: 0
Views: 438
Posted By causland
SPAdes duplicate contigs between chromosome and plasmid

Hi all,

Has anyone had any issues with SPAdes outputting duplicate sequences between plasmid and chromosome assembly outputs? I have an assembly ready to submit to NCBI but they won't take it...
Forum: RNA Sequencing 04-16-2019, 07:27 AM
Replies: 1
Views: 1,107
Posted By causland
HiSAT2 RNAseq alignment low + stranded data predicted to be unstranded

Hi all,

I have been receiving some confusing output with an RNA-seq dataset that I am trying to ultimately determine differential expression and fpkm values for.

Background: Library prep was...
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