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Forum: Sample Prep / Library Generation 09-03-2019, 06:00 PM
Replies: 15
Views: 2,641
Posted By firsal
Hi, I'm also doing Omni-ATAC-seq but I don't get...

Hi, I'm also doing Omni-ATAC-seq but I don't get reproducible results. Did you add 1x protease Inhibitor?
Forum: Sample Prep / Library Generation 08-07-2019, 08:17 PM
Replies: 9
Views: 3,162
Posted By firsal
Bioanalyser data of ATAC-seq library

Hi,

I have run ATAC-seq library preparation for the first time. I need advice about my Bioanalyser result. It seems the mono-nucleosomal ones are not in high concentration compared to multiple...
Forum: RNA Sequencing 10-06-2016, 12:29 AM
Replies: 3
Views: 1,133
Posted By firsal
Hi Conny, Thanks for your comments. I also...

Hi Conny,

Thanks for your comments. I also noticed the Mgcl2 is high as I used higher Mgcl2 in my smartseq2 protocol once and it didn't worked.

F
Forum: RNA Sequencing 09-20-2016, 01:07 AM
Replies: 3
Views: 1,133
Posted By firsal
G&T-seq

Hi,
I want to try G&T-seq protocol for sequencing RNA and DNA from one single cell. I have already experience in using Smart-seq 2 and MDA protocols.

But I'm interested to know if someone has...
Forum: RNA Sequencing 12-02-2015, 06:18 AM
Replies: 0
Views: 800
Posted By firsal
Question SNES, single nucleus exome sequencing method

Hi, Is it possible to use this method for whole genome sequencing not just for exome sequencing?

Also I wonder if it is easy, friendly use protocol.

I want to use this protocol from the...
Forum: Sample Prep / Library Generation 10-02-2014, 09:58 AM
Replies: 17
Views: 6,544
Posted By firsal
I'm sorry if I was not clear. I have frozen cells...

I'm sorry if I was not clear. I have frozen cells isolated from adipose tissue that I store them at -80 freezer. I freeze cells without any buffer. I do nuclei isolation from 5 ml of frozen cells by...
Forum: Sample Prep / Library Generation 10-01-2014, 10:47 PM
Replies: 17
Views: 6,544
Posted By firsal
Thanks for your comments. I'll try them. Could...

Thanks for your comments. I'll try them. Could be that I've isolated nuclei from frozen cells in -80?
Forum: Sample Prep / Library Generation 10-01-2014, 09:45 AM
Replies: 17
Views: 6,544
Posted By firsal
What does nuclear total RNA look like?



I enclosed the image of my result in attachment. It shows a wide peak of RNA from 100-2500 nucleotide.
Forum: Sample Prep / Library Generation 10-01-2014, 05:06 AM
Replies: 17
Views: 6,544
Posted By firsal
What does nuclear total RNA look like?

I ran it on nano chip. This is RNA isolated from 10,000 nuclei.
Forum: Sample Prep / Library Generation 10-01-2014, 04:13 AM
Replies: 17
Views: 6,544
Posted By firsal
What does nuclear total RNA look like?

Should I see the 18s and 28s peak when I running RNA BioAnalyser chips for Nuclei RNA? I don't see any peak in my run. Is that mean my nuclei RNA is degraded?
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