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Forum: Illumina/Solexa 06-28-2012, 02:55 AM
Replies: 0
Views: 994
Posted By ik76
outsourcing companies in Europe

Hi

Has anybody used ATLAS Biolabs, Fasteris, DNAVision or SourceBiosciences for exome or transcriptome sequencing? If so, any positive/negative comments?
Forum: Illumina/Solexa 01-05-2012, 05:50 AM
Replies: 1
Views: 1,100
Posted By ik76
HiSEQ flowcells/reagents compatibility

Hi

We would like to use HiSEQ v1.5 flowcell (prepared with v2.5 cluster generation kit) with HiSEQ v3 sequencing kit (lacking v2 sequencing reagents). Anybody has tried this? With what success?...
Forum: Service Providers 01-21-2011, 02:19 AM
Replies: 14
Views: 7,930
Posted By ik76
Reliable NGS bioinformatics service provider in Europe

Hi,
We are looking for a reliable bioinformatics service provider , preferably in Europe, for analysis of whole exome, target re-seq and RNA-seq data. Any recommendations?
Forum: Academic/Non-Profit Jobs 12-20-2010, 06:53 AM
Replies: 0
Views: 1,669
Posted By ik76
Senior Bioinformatics Officer NGS at the Institute of Cancer Research, London

We are seeking an experienced and motivated Senior Bioinformatics Officer to support Next Generation Sequencing (NGS) based projects within the Breakthrough Centre. The successful post holder will...
Forum: Sample Prep / Library Generation 03-22-2010, 05:45 AM
Replies: 1
Views: 1,440
Posted By ik76
4% agarose gels?

I was just wandering whether sopmebody have used 4% agarose gels instead of 2% for preparation of genomic or mRNA Illumina libraries.
Forum: Sample Prep / Library Generation 03-04-2010, 01:27 AM
Replies: 17
Views: 7,706
Posted By ik76
Hello, I was just wandering whether anybody...

Hello,
I was just wandering whether anybody have tried fragmenting total RNA using Covaris ( as a replacement for the fargmentation in zink solution recommnded in the Illumina mRNA sample prep...
Forum: Sample Prep / Library Generation 02-25-2010, 08:42 AM
Replies: 1
Views: 3,267
Posted By ik76
illumina alternative v1.5 protocol for small rna seq vs. the standard protocol

Hi All,
I was just wandering whether anybody had successfully used the illumina alternative v1.5 small RNA sample prep protocol . We tried but the only thing we could detect was adapter...
Forum: RNA Sequencing 02-09-2010, 07:46 AM
Replies: 0
Views: 1,319
Posted By ik76
problem with Illumina PE RNA seq alingment

Hi All,
We recently started to do PE RNA seq and we encountered a problem the cause of which have eluded us so far. In summary, following cycle 30 we see rapid increase in alignment error. The error...
Forum: Illumina/Solexa 01-08-2010, 04:31 AM
Replies: 2
Views: 2,009
Posted By ik76
questions about Illumina mRNA sample prep

Hi,
We are currently using a standard Illumina mRNA protocol and have experienced two problems :

i) some genomic contamination
ii) inefficient depletion (using the Ribo- kit)

Has somebody...
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