Forum: 454 Pyrosequencing
01-08-2009, 02:58 PM
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Replies: 61
Views: 93,847
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Forum: 454 Pyrosequencing
08-07-2008, 02:44 AM
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Replies: 61
Views: 93,847
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Forum: 454 Pyrosequencing
08-07-2008, 02:26 AM
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Replies: 61
Views: 93,847
Excellent! Thank you Siqusn. Those numbers are...
Excellent! Thank you Siqusn. Those numbers are informative for us. One last question, are you capturing a contiguous region (less Windowmasker repeatmasked regions)?
We are capturing a contiguous 5...
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Forum: 454 Pyrosequencing
08-06-2008, 06:46 PM
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Replies: 61
Views: 93,847
Hodges et al. reported that following something...
Hodges et al. reported that following something approximating your method they could get good Illumina GA-1 reads but suffered from poor sequence capture, whereas 500-mers could be captured OK but...
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Forum: 454 Pyrosequencing
08-06-2008, 01:49 AM
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Replies: 61
Views: 93,847
Hi Siqusn,
So you don't see a large decrease...
Hi Siqusn,
So you don't see a large decrease in the number of reads per flow cell lane like that reported by Hodges et al. (http://www.nature.com/ng/journal/v39/n12/abs/ng.2007.42.html)? I would...
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Forum: 454 Pyrosequencing
02-28-2008, 09:06 PM
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Replies: 61
Views: 93,847
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Forum: 454 Pyrosequencing
02-28-2008, 08:27 PM
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Replies: 61
Views: 93,847
Ultrasonicators
Yes. As I've found out either you, or your Next-gen service provider, needs a pretty funky new sonicator. It seems nebulisation won't cut it (bad pun :D) for 100 - 200 bp libraries. See these pieces...
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Forum: 454 Pyrosequencing
02-28-2008, 04:43 PM
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Replies: 61
Views: 93,847
Reseq: Many thanks for the info!
Horigen: No...
Reseq: Many thanks for the info!
Horigen: No one got back to me. But I have heard anecdotally that Nimblegen is on track to offer 385,000 probe chip enrichment as a service around the end of...
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Forum: 454 Pyrosequencing
01-29-2008, 02:23 PM
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Replies: 61
Views: 93,847
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Forum: 454 Pyrosequencing
01-29-2008, 02:07 PM
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Replies: 61
Views: 93,847
Okou et al.
Yes, you are quite right. Good spotting. I had become confused by the header on supplementary figure 1. 1 in 10 enrichment makes more sense and is in the same ballpark as Albert et al. Perhaps the...
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Forum: 454 Pyrosequencing
01-25-2008, 01:56 PM
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Replies: 61
Views: 93,847
Custom CGH
pmcget - It's my belief that Nimblegen will offer custom HD2 CGH chips around March.
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Forum: 454 Pyrosequencing
01-24-2008, 05:04 PM
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Replies: 61
Views: 93,847
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Forum: 454 Pyrosequencing
01-24-2008, 04:44 PM
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Replies: 61
Views: 93,847
My two cents...
I'm a postdoc employed to sequence a candidate region of interest via next-gen sequencing methods.
Here are my thoughts on Nimblegen capture as bullet points ripped from a presentation I gave to...
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