Forum: Illumina/Solexa
Yesterday, 07:41 PM
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Replies: 4
Views: 51
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Forum: Illumina/Solexa
Yesterday, 05:13 PM
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Replies: 4
Views: 51
Generally for human samples (3 gb genome) one...
Generally for human samples (3 gb genome) one would want to target around 25-30 Million reads per sample. So use that as an approximation to calculate the number of reads you would need per sample.
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Forum: Illumina/Solexa
03-04-2021, 07:02 AM
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Replies: 2
Views: 111
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Forum: Illumina/Solexa
03-01-2021, 05:52 AM
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Replies: 1
Views: 160
You should be able to run MiSeq nano FC (~1 M...
You should be able to run MiSeq nano FC (~1 M clusters) to check the balance of libraries and most of all to verify that libraries have worked. We routinely do that for pools destined for NovaSeq.
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Forum: Illumina/Solexa
02-10-2021, 12:22 PM
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Replies: 2
Views: 531
It appears to be about 2x faster compared to...
It appears to be about 2x faster compared to bcl2fastq on identical datasets. It is producing different folder structure for result/log files (no more html reports) but plain csv so they should be...
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Forum: Bioinformatics
02-04-2021, 05:06 PM
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Replies: 7
Views: 3,258
BBMap suite programs try to auto detect memory...
BBMap suite programs try to auto detect memory available on your system. If they are not able to do that they will fall back to using a safe 2GB using the option mentioned "-Xmx2000m". This is Java...
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Forum: Bioinformatics
02-03-2021, 12:35 PM
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Replies: 39
Views: 33,262
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Forum: Bioinformatics
01-31-2021, 02:08 PM
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Replies: 1
Views: 561
If these are prokarotic genomes them Mauve...
If these are prokarotic genomes them Mauve (http://darlinglab.org/mauve/mauve.html) will work. If these are larger eukaryotic genomes them tools like Mugsy (http://mugsy.sourceforge.net/) or MUMMER4...
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Forum: Bioinformatics
01-26-2021, 05:53 AM
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Replies: 4
Views: 631
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Forum: Illumina/Solexa
01-25-2021, 10:20 AM
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Replies: 3
Views: 666
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Forum: Illumina/Solexa
01-25-2021, 04:27 AM
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Replies: 3
Views: 666
Why is your facility not doing the...
Why is your facility not doing the demultiplexing? This should be very simple for them to do.
It is normal to have a few reads at the beginning of the file to have some N's but are you saying...
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Forum: Bioinformatics
11-29-2020, 05:47 PM
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Replies: 1
Views: 678
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Forum: Illumina/Solexa
11-18-2020, 03:52 AM
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Replies: 4
Views: 691
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Forum: Illumina/Solexa
11-07-2020, 06:49 AM
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Replies: 1
Views: 978
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Forum: Bioinformatics
09-29-2020, 03:30 AM
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Replies: 2
Views: 1,139
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Forum: Bioinformatics
09-19-2020, 05:44 AM
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Replies: 5
Views: 1,379
Create a ticket...
Create a ticket (https://sourceforge.net/projects/bbmap/support) via BBMap's repo so Brian is aware of the problem.
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Forum: Bioinformatics
09-17-2020, 09:42 AM
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Replies: 5
Views: 1,379
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Forum: RNA Sequencing
09-15-2020, 07:16 PM
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Replies: 6
Views: 1,971
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Forum: General
09-15-2020, 04:02 AM
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Replies: 2
Views: 1,905
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Forum: RNA Sequencing
09-14-2020, 11:37 AM
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Replies: 6
Views: 1,971
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Forum: Bioinformatics
09-10-2020, 11:37 AM
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Replies: 5
Views: 1,379
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Forum: Bioinformatics
09-04-2020, 10:09 AM
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Replies: 2
Views: 1,882
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Forum: Illumina/Solexa
09-02-2020, 04:07 AM
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Replies: 8
Views: 3,470
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Forum: Bioinformatics
09-01-2020, 10:58 AM
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Replies: 4
Views: 966
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Forum: Bioinformatics
09-01-2020, 03:51 AM
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Replies: 4
Views: 966
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