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Forum: 454 Pyrosequencing 01-28-2008, 11:59 AM
Replies: 61
Views: 93,847
Posted By colicoli
slow hybs in array capture methods

My guess for the reason for the long hybs to Nimblegen arrays is that at 42 degrees, it takes a LONG time for the hybridization reaction to approach equilibrium. While short oligos can hyb quickly...
Forum: 454 Pyrosequencing 12-13-2007, 09:41 AM
Replies: 61
Views: 93,847
Posted By colicoli
Target enrichment methods

Two points about the recently published target enrichment methods.

1. Comparing the methods from the different papers is not easy. First, Okou et al. used a resequencing array, which gives no...
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