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Forum: Illumina/Solexa 12-21-2010, 12:52 PM
Replies: 4
Views: 3,561
Posted By spadejac
Qseq is what would be common between different...

Qseq is what would be common between different Illumina systems, but one could configure to get fastq or Illumina native(previously called SCARF) from the qseq files. Qseq's will generally be too...
Forum: Illumina/Solexa 12-21-2010, 12:36 PM
Replies: 9
Views: 6,650
Posted By spadejac
No other explanations. Here is NCBI documentation...

No other explanations. Here is NCBI documentation about it:

SRR000001.fastq – Fragment library data, or unpaired mates from a paired library.
SRR000001_1.fastq – First mate sequence....
Forum: Bioinformatics 09-27-2009, 04:38 PM
Replies: 21
Views: 34,122
Posted By spadejac
Bowtie for alignment

Oh yeah! We have. And that is the best that I've come across in my career for alignment of short reads. Just too fast - Great for expression data.

Spade
Forum: Introductions 09-27-2009, 04:35 PM
Replies: 373
Views: 148,935
Posted By spadejac
Hi

Hi guys,

I work in the academia. My area of focus is analysis of short reads of SBS sequencing using Illumina GAI-II. I am experienced with the old-generation MPSS & 454. Most of my job...
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