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Search: Posts Made By: Bukowski
Forum: Bioinformatics 02-10-2018, 04:42 PM
Replies: 3
Views: 521
Posted By Bukowski
It's down. https://gitlab.com/ezlab/busco ...

It's down.

https://gitlab.com/ezlab/busco

You can find links to the code and datasets there.
Forum: Bioinformatics 01-22-2018, 01:58 AM
Replies: 14
Views: 761
Posted By Bukowski
What was the goal of sequencing them in the first...

What was the goal of sequencing them in the first place? This might inform any potential answers...
Forum: Ion Torrent 01-17-2018, 04:58 AM
Replies: 4
Views: 487
Posted By Bukowski
'Deblur has not been evaluated with Ion Torrent':...

'Deblur has not been evaluated with Ion Torrent': https://forum.qiime2.org/t/deblur-p-trim-length-deactivation-failure/1880/5

DADA2 recommendations for Ion Torrent data:...
Forum: Illumina/Solexa 12-19-2017, 04:44 AM
Replies: 2
Views: 530
Posted By Bukowski
https://support.illumina.com/downloads/sequencing_...

https://support.illumina.com/downloads/sequencing_coverage_calculator.html
Forum: Bioinformatics 11-09-2017, 04:40 AM
Replies: 2
Views: 432
Posted By Bukowski
https://www.biostars.org/p/109425/

https://www.biostars.org/p/109425/
Forum: Academic/Non-Profit Jobs 10-12-2017, 08:58 AM
Replies: 0
Views: 311
Posted By Bukowski
Research Fellow in Microbial Genomics, Aberdeen - Sctoland

Posting on behalf of a colleague:

REF: SBS028R

We seek a highly motivated and interactive Microbial Genomicist to take a leading role in a Natural Environment Research Council-funded project...
Forum: Academic/Non-Profit Jobs 08-07-2017, 02:34 AM
Replies: 0
Views: 287
Posted By Bukowski
Genomics Pipeline Group Software Developer - Earlham Institute, Norwich UK

We currently have a job open in the Genomics Pipelines Group for a Software Developer. Please note this is not a bioinformatics post per se, but will work closely with bioinformaticians and others in...
Forum: Industry Jobs! 08-02-2017, 06:30 AM
Replies: 0
Views: 3,734
Posted By Bukowski
Bioinformatician/statistical genetcisist - Cytox Ltd (UK)

I'm not posting this on behalf of myself, but for a company I worked with on a project a couple of years ago who I'm prepared to vouch for in terms of how interesting I think the role might be for...
Forum: Bioinformatics 05-19-2017, 04:29 AM
Replies: 12
Views: 968
Posted By Bukowski
How's your lawsuit going? Question is...

How's your lawsuit going?

Question is rhetorical. I've met far more people who allege malpractice of this type in the industry, than seen malpractice of this type in the industry...
Forum: Pacific Biosciences 05-10-2017, 12:56 AM
Replies: 5
Views: 1,619
Posted By Bukowski
The Sequel consumables starter kit, which...

The Sequel consumables starter kit, which provides enough materials for 16 cells is 20k - admittedly this is not the cheapest way to buy consumables, but is closer to 1.25k/cell.
Forum: Pacific Biosciences 05-09-2017, 11:27 AM
Replies: 5
Views: 1,619
Posted By Bukowski
1) Accuracy of >99.999% - Yes this will be...

1) Accuracy of >99.999%

- Yes this will be consensus accuracy at depth.

2) Output of ~5Gb per SMRT cell / ~800 per SMRT cell run

- That's about what we're seeing. Without going too far...
Forum: Bioinformatics 04-07-2017, 05:00 AM
Replies: 3
Views: 567
Posted By Bukowski
http://biorxiv.org/content/early/2016/08/12/068809

http://biorxiv.org/content/early/2016/08/12/068809
Forum: Bioinformatics 03-31-2017, 12:09 PM
Replies: 10
Views: 1,609
Posted By Bukowski
You're looking at the wrong genome...

You're looking at the wrong genome build/annotation in IGV almost certainly.
Forum: Illumina/Solexa 03-23-2017, 02:50 AM
Replies: 29
Views: 4,884
Posted By Bukowski
So.. no this issue is not resolved. I have an...

So.. no this issue is not resolved. I have an email from Illumina today which I will summarise.

The issue is that there is a byproduct in their manufacturing process of fluorescent nucleotides....
Forum: Illumina/Solexa 03-02-2017, 09:29 AM
Replies: 8
Views: 1,503
Posted By Bukowski
That library prep is about as simple as it gets....

That library prep is about as simple as it gets. What you need is automation, not a different method.
Forum: Illumina/Solexa 03-02-2017, 04:11 AM
Replies: 5
Views: 842
Posted By Bukowski
Does this imply you did pre-capture pooling? It...

Does this imply you did pre-capture pooling? It could be that post-capture PCR has had to have a few extra rounds, perhaps the hybridisations didn't yield well enough - that will increase your...
Forum: Bioinformatics 03-01-2017, 09:20 AM
Replies: 3
Views: 566
Posted By Bukowski
Try antismash:...

Try antismash: http://antismash.secondarymetabolites.org/
Forum: Academic/Non-Profit Jobs 03-01-2017, 04:35 AM
Replies: 0
Views: 424
Posted By Bukowski
Bioinformatician (maternity cover) at Earlham Institute, Norwich, UK

http://eridanus.net/index.png

The Platforms and Pipelines (P&P) Group at Earlham is responsible for delivering the BBSRC National Capability in Genomics (NCG), providing access to state of the art...
Forum: Core Facilities 02-25-2017, 04:46 AM
Replies: 1
Views: 1,132
Posted By Bukowski
Correct bit.ly is http://bit.ly/2lJyjhC

Correct bit.ly is http://bit.ly/2lJyjhC
Forum: Illumina/Solexa 02-10-2017, 10:46 AM
Replies: 2
Views: 664
Posted By Bukowski
Are you paying for the sequencing?

Are you paying for the sequencing?
Forum: Pacific Biosciences 09-06-2016, 06:19 AM
Replies: 3
Views: 871
Posted By Bukowski
http://www.slimsuite.unsw.edu.au/servers/pacbio.ph...

http://www.slimsuite.unsw.edu.au/servers/pacbio.php is one solution.
Forum: Pacific Biosciences 09-06-2016, 04:39 AM
Replies: 3
Views: 871
Posted By Bukowski
http://www.pacb.com/smrt-science/smrt-sequencing/a...

http://www.pacb.com/smrt-science/smrt-sequencing/accuracy/ - you do not have enough coverage for 'perfect consensus accuracy'
Forum: Bioinformatics 09-06-2016, 04:27 AM
Replies: 15
Views: 1,923
Posted By Bukowski
Citation for this please?

Citation for this please?
Forum: Bioinformatics 08-23-2016, 08:24 AM
Replies: 4
Views: 1,318
Posted By Bukowski
I have an Opgen Argus, and I've just checked -...

I have an Opgen Argus, and I've just checked - the CD-ROM comes with a time-limited activation key, and we can't even install it on our own laptops anymore (even though it's working on the machine...
Forum: Bioinformatics 08-22-2016, 04:42 AM
Replies: 4
Views: 1,318
Posted By Bukowski
This might be due to the fact they're...

This might be due to the fact they're discontinuing the platform, it's already all but erased from their website. Perhaps you need to get access to it via someone who already owns the system in an...
Showing results 1 to 25 of 344

 


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