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Forum: Bioinformatics 07-01-2015, 11:39 PM
Replies: 211
Views: 50,581
Posted By fdts
Wonderful, thanks!

Wonderful, thanks!
Forum: Bioinformatics 07-01-2015, 05:50 AM
Replies: 211
Views: 50,581
Posted By fdts
Just a comment, not sure if this is the place: ...

Just a comment, not sure if this is the place:
It would be helpful for downstream analysis if the refstat files that can be output using bbsplit produced 0s (zeros) for references that have no reads...
Forum: Bioinformatics 05-20-2015, 12:27 AM
Replies: 211
Views: 50,581
Posted By fdts
Aah, I see. Thanks to both of you. Would these...

Aah, I see. Thanks to both of you. Would these flags work with BBDuk then?

Data quality is decent. I will map to a de novo metagenome assembly generated from the same data. I am kind of hopeful to...
Forum: Bioinformatics 05-19-2015, 03:11 AM
Replies: 211
Views: 50,581
Posted By fdts
quality filtering

Do you recommend quality trimming before mapping using BBMap? I am talking illumina 100bp PE.
I have been searching the forum for a while but could not find a hint.

Thanks
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