SEQanswers

Go Back   SEQanswers > Search Forums


Showing results 1 to 6 of 6
Search took 0.00 seconds.
Search: Posts Made By: ian Adams
Forum: 454 Pyrosequencing 01-07-2011, 03:46 AM
Replies: 3
Views: 3,078
Posted By ian Adams
My understanding is that Solexa read length is...

My understanding is that Solexa read length is limited as the flurochromes interfer with the DNA polymerase reducing its efficiency and thus eventually a majority of the strands have been terminated...
Forum: 454 Pyrosequencing 06-24-2010, 01:55 AM
Replies: 10
Views: 2,432
Posted By ian Adams
yes I would expect that you have your raw reads...

yes I would expect that you have your raw reads in the files "Stressed_454Reads.zip" and "Control_454Reads.zip" probably as .fna and .qual
The .fna file is the sequences in .fasta format and the...
Forum: 454 Pyrosequencing 06-21-2010, 08:52 AM
Replies: 42
Views: 11,957
Posted By ian Adams
Sorry for the delay, been at the 454 user meeting...

Sorry for the delay, been at the 454 user meeting
Tried all three, various settings for B and C gave me between 150-500 sequences (and notice the absence of the K!). A (using the shotgun protocol)...
Forum: 454 Pyrosequencing 06-10-2010, 02:43 AM
Replies: 42
Views: 11,957
Posted By ian Adams
We've had similar problems running amplicons,...

We've had similar problems running amplicons, (Lib-A with only A primer) our first run had high short quantity (90%). We ran a bioanalyser chip on the post ampure amplicons and noticed a smaller...
Forum: Service Providers 01-13-2009, 01:29 PM
Replies: 162
Views: 137,139
Posted By ian Adams
A few more sequencing services (UK)

I use Liverpool university and have had good results, I've also found a couple more universities who have recently got 454 sequencers:


York University Technology Facility
...
Forum: 454 Pyrosequencing 11-04-2008, 09:59 AM
Replies: 9
Views: 6,921
Posted By ian Adams
454 homopolymer errors or????

I have some 454 data produced from cDNA randomly amplified using proofreading taq, sheared and then sequenced. Once the amplification tags were removed, we'ved assembled it using CLC genomic work...
Showing results 1 to 6 of 6

 


All times are GMT -8. The time now is 10:49 PM.


Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2018, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO