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Forum: Sample Prep / Library Generation 06-27-2014, 02:34 AM
Replies: 4
Views: 1,371
Posted By elenstea
yes I am aiming for larger fragments! thanks for...

yes I am aiming for larger fragments! thanks for the info!
Forum: Sample Prep / Library Generation 06-27-2014, 02:19 AM
Replies: 4
Views: 1,371
Posted By elenstea
I am sorry I meant decreasing TDE!!!!!!!!!!!!!!!!

I am sorry I meant decreasing TDE!!!!!!!!!!!!!!!!
Forum: Sample Prep / Library Generation 06-27-2014, 01:04 AM
Replies: 4
Views: 1,371
Posted By elenstea
Nextera modification

Hello everyone, has anyone tried to modify the amount of tranposome during tagmantation using Nextera? We have already modified the protocol with good results concerning tagmentation time, but we are...
Forum: Sample Prep / Library Generation 06-26-2014, 11:52 PM
Replies: 3
Views: 2,584
Posted By elenstea
We had the same problem several time, we stopped...

We had the same problem several time, we stopped using beads, now using spin columns and we have no problem
Forum: Sample Prep / Library Generation 06-26-2014, 11:30 PM
Replies: 2
Views: 2,456
Posted By elenstea
How long do you let the pellet dry? And how much...

How long do you let the pellet dry? And how much buffer do you use for resuspending? I would suggest close monitoring of the pellet while drying (not enough drying and pellet will not dissolve...
Forum: Illumina/Solexa 05-30-2014, 05:37 AM
Replies: 2
Views: 1,770
Posted By elenstea
thank you :)

thank you :)
Forum: Illumina/Solexa 05-30-2014, 03:49 AM
Replies: 2
Views: 1,770
Posted By elenstea
Question MiSeq newbie / Library prep questions

Hello everyone. I am using MiSeq from Illumina using NEXTERA DNA kit. I have already performed 2 runs (first run with 2X150 v.2 went super great :) , the second run with 2X75 v.3 which was not so...
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