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Forum: Sample Prep / Library Generation 08-05-2017, 09:57 AM
Replies: 2
Views: 2,463
Posted By barn88
Thanks for your response. I had considered...

Thanks for your response. I had considered modifying the ddRAD P2 adapter; I just wanted to see what suggestions other people made.
Forum: Sample Prep / Library Generation 07-28-2017, 07:26 AM
Replies: 2
Views: 2,463
Posted By barn88
Questions about RADseq oligo

I will be genotpying ~500 samples using the original RADseq protocol, and I have a couple of questions.

First, is desalting a sufficient purification method for the barcoded adapters? I plan to...
Forum: Sample Prep / Library Generation 05-24-2017, 08:29 AM
Replies: 2
Views: 1,309
Posted By barn88
Good point, not sure how I overlooked that. ...

Good point, not sure how I overlooked that. Thanks!
Forum: Sample Prep / Library Generation 05-08-2017, 09:44 AM
Replies: 2
Views: 1,309
Posted By barn88
Need feedback on RADseq using enzyme BbvCI

I am planning to genotype ~520 samples using original RADseq (Etter et al. 2011). The project has 2 goals: (1) construct a linkage map using a subset of those individuals, and (2) examine...
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