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Forum: Sample Prep / Library Generation 10-18-2016, 12:01 PM
Replies: 2
Views: 2,928
Posted By aubrie
Does anyone have experience with automating this...

Does anyone have experience with automating this kit on the EpMotion?

Thank you,
Aubrie
Forum: Sample Prep / Library Generation 10-13-2016, 08:57 AM
Replies: 29
Views: 7,122
Posted By aubrie
I'm thinking about putting the rRNA depleted...

I'm thinking about putting the rRNA depleted sample through the RNeasy clean up to get rid of the small RNAs below 200bp. I have sample to spare and I'm curious to see the concentration of "mRNA"...
Forum: Sample Prep / Library Generation 10-12-2016, 11:00 AM
Replies: 29
Views: 7,122
Posted By aubrie
The concentrations for the samples (including the...

The concentrations for the samples (including the monster peak) are 20-24ng/ul in 16ul.

I did not run a negative control. If I did I assume this would be an amount of the tRNA with beads and no...
Forum: Sample Prep / Library Generation 10-12-2016, 09:34 AM
Replies: 29
Views: 7,122
Posted By aubrie
Assistance please

4542

4543Hi,

I am working with RNA from e.coli k12 strain. I have flash frozen the samples and then stored them in the minus 80, there is a control and then treatments in triplicate with...
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