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Forum: Sample Prep / Library Generation 12-21-2015, 01:18 PM
Replies: 289
Views: 154,472
Posted By longwood
Superscript II contamination update

E. Coli contamination of superscript II has been confirmed by the manufacturer. I know a bunch of people reported this on this forum a few months back, so thought I'd share this letter (attached) I...
Forum: Sample Prep / Library Generation 08-17-2015, 08:43 AM
Replies: 289
Views: 154,472
Posted By longwood
Extremely helpful information Simone78 and...

Extremely helpful information Simone78 and nucacidhunter! Thank you very much!
Forum: Sample Prep / Library Generation 08-14-2015, 10:59 AM
Replies: 289
Views: 154,472
Posted By longwood
Has anyone tried using homebrew barcodes for...

Has anyone tried using homebrew barcodes for multiplexing? Or just ordered the oligos (as listed in the Illumina Customer Sequence Letter) from an oligo vendor instead of ordering the expensive index...
Forum: Sample Prep / Library Generation 07-24-2015, 11:35 AM
Replies: 289
Views: 154,472
Posted By longwood
I'm still learning the ABCs of RNAseq analysis so...

I'm still learning the ABCs of RNAseq analysis so pardon me if I sound ignorant but if the goal is quantitative accuracy of gene expression (to the extent that the 5' end of an mRNA faithfully...
Forum: Sample Prep / Library Generation 07-08-2015, 09:10 AM
Replies: 289
Views: 154,472
Posted By longwood
Thanks everyone for sharing your SSII troubles. ...

Thanks everyone for sharing your SSII troubles.

I also got bad results with a recently acquired batch of Superscript II; an older batch was working fine. Good: 1631423; Bad: 1685467.
...
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