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Forum: Epigenetics 09-10-2019, 12:41 AM
Replies: 0
Views: 1,066
Posted By plefebvre
Diagenode PiocRuptor

Hi there,
I would like to get any feedback on your experience with Diagenode PicoRuptor. Our is performing poorly and customer service is at best not helpful. Thanks for your time.
Forum: RNA Sequencing 10-03-2012, 06:57 AM
Replies: 6
Views: 2,366
Posted By plefebvre
you definitively would not see miRNA in your...

you definitively would not see miRNA in your RNA-seq data
Forum: Bioinformatics 10-03-2012, 06:49 AM
Replies: 8
Views: 1,752
Posted By plefebvre
may be start all over using a good DNAseI??

may be start all over using a good DNAseI??
Forum: Bioinformatics 10-03-2012, 06:44 AM
Replies: 2
Views: 1,016
Posted By plefebvre
yes, same applies to the end of transcripts......

yes, same applies to the end of transcripts... dont look for TGA :D
Forum: Bioinformatics 04-02-2012, 08:59 AM
Replies: 2
Views: 2,050
Posted By plefebvre
Worked nicely, until 17:57:40 <INFO> The...

Worked nicely, until

17:57:40 <INFO> The total count of short reads mapped on exons is : 197807
17:57:40 <INFO> Parsing BAM file is complete.
17:57:40 <INFO> Start building coverage file...
Forum: Bioinformatics 03-31-2012, 10:06 AM
Replies: 2
Views: 2,050
Posted By plefebvre
Bam to Wig

Hi bioinformatics wiz
I am looking for a simple way to convert sam/bam to wig files. Does any one knows of a simple tool I may use for that (please no Unix nor Python command lines!)
Thanks
Forum: Bioinformatics 05-09-2011, 02:02 AM
Replies: 5
Views: 2,496
Posted By plefebvre
Thanks, I guess you are referring to the...

Thanks, I guess you are referring to the "mygenomatix" software suite?
Forum: Bioinformatics 05-08-2011, 12:23 PM
Replies: 5
Views: 2,496
Posted By plefebvre
Cuffdiff and graphics

Hi everybody
Now that I could get (painfully ;-)) through the whole analysis of our RNA-seq data using Galaxy, I am quite puzzled on how produced some decent and informative graphic information...
Forum: RNA Sequencing 04-20-2011, 01:49 AM
Replies: 1
Views: 1,417
Posted By plefebvre
Cufflinks then Cuffdiff

Hi there
I have ran a RNA-seq on Illumina GII, and processed the bam files on the Galaxy server. Everything ran smoothly untill I tried to run Cuffdiff on may assembled transcript files which...
Forum: Introductions 01-30-2010, 07:33 AM
Replies: 0
Views: 1,078
Posted By plefebvre
Hi there

Hello everybody
Getting our first Chip seq data results, and thus getting into trouble to analyze them properly :D
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