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Forum: Illumina/Solexa 05-17-2011, 01:37 PM
Replies: 17
Views: 6,205
Posted By SMO
What kind of cluster densities do you see with...

What kind of cluster densities do you see with 100-130million reads? How many pM are you loading to get that number of reads?
Forum: Genomic Resequencing 05-05-2011, 05:21 AM
Replies: 9
Views: 4,249
Posted By SMO
Thanks for the input. Do you see similar...

Thanks for the input.
Do you see similar coverage plots for Agilent mouse?
We have noticed that the curves for mouse differ greatly from human, using the same protocol, reagents and technicians. ...
Forum: Genomic Resequencing 05-04-2011, 06:18 AM
Replies: 9
Views: 4,249
Posted By SMO
Also, we are getting an average of 80 million...

Also, we are getting an average of 80 million reads for a PE 2x101.
Forum: Genomic Resequencing 05-03-2011, 05:34 AM
Replies: 9
Views: 4,249
Posted By SMO
on target is around 72-80%.

on target is around 72-80%.
Forum: Genomic Resequencing 05-02-2011, 11:52 AM
Replies: 9
Views: 4,249
Posted By SMO
These were ran on HiSeq 2000, and were not...

These were ran on HiSeq 2000, and were not indexed.
Forum: Genomic Resequencing 05-02-2011, 11:27 AM
Replies: 9
Views: 4,249
Posted By SMO
Agilent human exon coverage plots

My lab has been using the Agilent 50Mb SureSelect Human All Exon kit for many months now. Though the data is acceptable, we canít help but wonder why our coverage plots look so different from...
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