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Forum: Illumina/Solexa 07-14-2016, 09:50 AM
Replies: 3
Views: 1,603
Posted By JoeChris38
Custom multiplex sequencing barcodes / indexes (8-mer)

Hi all,

I have synthesised a custom set of Illumina adaptors that use an 8-mer index to allow 384-plex multiplexing. I got these index sequences from the Nextera XT 384-plex index kit.

I am...
Forum: Genomic Resequencing 02-05-2014, 09:04 AM
Replies: 2
Views: 2,160
Posted By JoeChris38
Thanks, that's really useful. I noted this...

Thanks, that's really useful.

I noted this paper: http://www.ncbi.nlm.nih.gov/pubmed/?term=hite+pcr+amplification showing that reduced temperature appeared to reduce stutter. Have you tried any...
Forum: Genomic Resequencing 01-27-2014, 09:32 AM
Replies: 2
Views: 2,160
Posted By JoeChris38
PCR amplifying microsatellites (short tandem repeats) for sequencing

Hi all,

I'm looking to amplify specific dinucleotide repeats from low input DNA (~1ng) but I'm having problems with microsatellite contraction/expansion during rounds of PCR. Has anyone with...
Forum: Pacific Biosciences 10-11-2013, 07:03 AM
Replies: 1
Views: 3,852
Posted By JoeChris38
PacBio RS II Library Prep Input

Hi all,

I'm looking to sequence low quantities of PCR amplicon using the PacBio RS II system. I am trying to detect single nucleotide variants within these amplicons so need to minimise the number...
Forum: General 08-02-2013, 03:53 AM
Replies: 5
Views: 2,321
Posted By JoeChris38
I have thought about this option but I think my...

I have thought about this option but I think my lack of reproducibility from the primer pool is as a result of the primer pool itself and not due to the low template input. Though I will be...
Forum: General 08-01-2013, 06:56 AM
Replies: 5
Views: 2,321
Posted By JoeChris38
Targeted sequencing of ~40Mb genome

I am looking to do targeted sequencing on approximately ~40Mb of mouse genome (~2% of the genome) from low input DNA (~100pg). Equivalent to sequencing the equivalent of 2 exomes worth of DNA from 20...
Forum: Illumina/Solexa 04-04-2013, 06:42 AM
Replies: 5
Views: 4,391
Posted By JoeChris38
Nextera XT Normalisation

Hi all,

I have been using the Nextera XT kit to do my library preps for MiSeq sequencing of fairly low complexity libraries and have been getting very variable results as a result of variable...
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