Forum: Sample Prep / Library Generation
06-27-2019, 11:05 AM
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Replies: 5
Views: 3,699
BioNano
I agree with Phillip. Use the agarose plugs for the lysis. It might be old school, but it is making a comeback! You can use the BioNano protocol for high MW DNA. It's one of the best protocols...
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Forum: De novo discovery
06-27-2019, 10:55 AM
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Replies: 1
Views: 3,783
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Forum: Bioinformatics
04-30-2019, 04:50 AM
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Replies: 12
Views: 3,098
Canine genomes
I liked GenoMax's suggestion, so go with that if you haven't already. Also be sure to use long read (PacBio) based assembly for your analysis against a reference genome.
If you are interested in...
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Forum: Sample Prep / Library Generation
04-24-2019, 11:53 AM
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Replies: 1
Views: 1,451
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Forum: De novo discovery
04-09-2019, 07:09 AM
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Replies: 2
Views: 7,719
Hi Pawan,
I also would highly recommend...
Hi Pawan,
I also would highly recommend Phase Genomics' HiC approach to getting telomere and centromere information. Blasting for it may be tricky since those portions generally don't assemble...
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Forum: De novo discovery
04-09-2019, 06:59 AM
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Replies: 3
Views: 5,831
Scaffolding
As nucacidhunter mentioned, I would highly recommend you work with the VGP group. I've work with the VGP and had excellent results for my de novo projects. They even do the hybrid assemblies!
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