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Forum: Sample Prep / Library Generation 04-06-2017, 02:19 AM
Replies: 5
Views: 2,019
Posted By Amcb1
Thank you GA-J, It's possible as most of the...

Thank you GA-J,
It's possible as most of the extra bands are present in samples that had the higher concentration of starting material, however it should have been normalized to 500ng per sample...
Forum: Sample Prep / Library Generation 04-04-2017, 02:19 AM
Replies: 5
Views: 2,019
Posted By Amcb1
Thank you nucacidhunter, I guess you are...

Thank you nucacidhunter,

I guess you are probably correct, if so I will see this when I carryout the test sequencing on the Miseq. You are also correct re. the pre and post PCR area at present...
Forum: Sample Prep / Library Generation 04-03-2017, 10:29 AM
Replies: 5
Views: 2,019
Posted By Amcb1
Unexpected 425bp band un Truseq stranded mRNA library prep

Hello,
I joined the community earlier today and I have a problem, I have carried out a Truseq Stranded mRNA HT library prep. and have an unexpected band in some of the samples. I used half of the...
Forum: Introductions 04-03-2017, 03:55 AM
Replies: 0
Views: 852
Posted By Amcb1
Smile Good day to all

Hello,

I have been working in NSG for about 18 months and am running a Nextseq and carrying out various library preps, gaining experience as I go. Mostly all has gone well but from time to time I...
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