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Forum: Illumina/Solexa 02-24-2016, 01:48 PM
Replies: 4
Views: 1,963
Posted By niroshan
1) Yes, these are you region specific primers,...

1) Yes, these are you region specific primers, adjacent to your region of interest

2) Adaptors are the portion of your library read fragment that will attach to the flow cell surface. The flow...
Forum: Illumina/Solexa 01-27-2016, 02:53 PM
Replies: 10
Views: 3,054
Posted By niroshan
You should be able to find the sequences in this...

You should be able to find the sequences in this Document:...
Forum: The Pipeline 01-14-2016, 12:49 PM
Replies: 16
Views: 14,340
Posted By niroshan
NanoString Developing Novel Sequencing Chemistry Based on Optical Barcoding

At the JP Morgan Healthcare Conference, NanoString Technologies CEO Brad Gray said that the firm has been developing a novel single-molecule hybridization-based sequencing chemistry that leverages...
Forum: Illumina/Solexa 12-18-2015, 10:07 AM
Replies: 2
Views: 1,678
Posted By niroshan
Recommended washes are outlined in the MiSeq User...

Recommended washes are outlined in the MiSeq User Guide...
Forum: Illumina/Solexa 11-25-2015, 03:23 PM
Replies: 2
Views: 2,013
Posted By niroshan
Do you have access to the MiSeq Reporter? You...

Do you have access to the MiSeq Reporter? You should be able to access it by opening the browser on your Miseq and typing http://localhost:8042/ in the addressbar bar.
If you have specified...
Forum: Illumina/Solexa 11-20-2015, 11:13 AM
Replies: 6
Views: 2,269
Posted By niroshan
Illumina uses two lasers (green: G/T and red:...

Illumina uses two lasers (green: G/T and red: A/C) to sequence. It is important to maintain color balance for each base of the read being sequenced, otherwise you'll run in difficulties for accurate...
Forum: 454 Pyrosequencing 11-20-2015, 10:54 AM
Replies: 7
Views: 8,941
Posted By niroshan
Yes, it is compatible with all major platforms ...

Yes, it is compatible with all major platforms 454; Illumina HiSeq, and MiSeq; and Ion Torrent PGM.
Forum: Bioinformatics 02-23-2015, 12:40 PM
Replies: 1
Views: 841
Posted By niroshan
You can download the complete curated COSMIC...

You can download the complete curated COSMIC dataset here (registration needed):
http://cancer.sanger.ac.uk/cancergenome/projects/cosmic/download
Forum: The Pipeline 02-09-2015, 01:42 PM
Replies: 16
Views: 14,340
Posted By niroshan
Q-SEQ from QuantumDX Alongside QuantuMDx's...

Q-SEQ from QuantumDX

Alongside QuantuMDx's novel sequencing by synthesis (SBS) DNA sequencing chemistry deployed on its nanowire FET biosensors, QMDx is also developing a novel DNA sequencing...
Forum: Illumina/Solexa 10-20-2014, 10:02 AM
Replies: 3
Views: 1,254
Posted By niroshan
This may help you. Go to this page: ...

This may help you. Go to this page:
http://support.illumina.com/training/online-courses/sequencing.html

and click on "MiSeq: Does My Run Look Good?"
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