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Forum: Sample Prep / Library Generation 04-15-2017, 12:18 PM
Replies: 1
Views: 2,492
Posted By zhaolin
I keep the PCR product in 4 degree after 5xcycles...

I keep the PCR product in 4 degree after 5xcycles pre-run. After extension cycles, I purified the library and stored them in minus 20 degree. I prefer keep them on ice rather than RT although samples...
Forum: Sample Prep / Library Generation 11-17-2014, 09:00 AM
Replies: 9
Views: 5,661
Posted By zhaolin
What is the size of your library smear? I have...

What is the size of your library smear? I have tried to lower the transposase amount in the reaction and found the size of PCR product changes. But it still a piece of smear. How much of PCR product...
Forum: Sample Prep / Library Generation 11-14-2014, 07:09 AM
Replies: 9
Views: 5,661
Posted By zhaolin
I haven't start doing the gel checking. But I...

I haven't start doing the gel checking. But I would change the transposase digestion time if I got a smear.
Forum: Sample Prep / Library Generation 11-13-2014, 11:52 AM
Replies: 9
Views: 5,661
Posted By zhaolin
Rn/cycles: qPCR decided ATAC-seq library amplification

Hello, I have tried to set up a experiment following the ATAC-seq protocol. when I ran the qPCR side reaction to decide the cycle for library amplification, I met the problem.

The protocol uses...
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