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Forum: Illumina/Solexa 09-03-2017, 01:53 PM
Replies: 9
Views: 1,559
Posted By dlin82
Thanks for the quick reply nucacidhunter! ...

Thanks for the quick reply nucacidhunter!

Yes it's homebrew so I guess I'll start with 20-30% PhiX spike-in. I am reading the Meissner mRRBS paper more in detail and noticed they do dark cycles....
Forum: Illumina/Solexa 09-03-2017, 12:58 PM
Replies: 9
Views: 1,559
Posted By dlin82
Hi - sorry to revive an old thread, we are...

Hi - sorry to revive an old thread, we are gearing up our own very first RRBS run on the NextSeq500 so wanted to check in if 30% is actually a good amount of PhiX to spike in. Are the quality of...
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