Forum: General
04-02-2020, 06:43 AM
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Replies: 4
Views: 4,894
Hi Rnasoup,
Thanks for your reply. What I...
Hi Rnasoup,
Thanks for your reply. What I meant is the data quality in terms of reported phred score vs observed phred score, substitution/indel error rate, problematic region for variant calling...
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Forum: General
03-27-2020, 03:18 AM
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Replies: 4
Views: 4,894
Hi,
Thanks Rnasoup for sharing the link to...
Hi,
Thanks Rnasoup for sharing the link to the sequencing adapter. I tried to find it but nothing showed up in google.
I hope jgroh resolved the adapter issue. It does look quite strange.
By...
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Forum: Vendor Forum
03-12-2020, 03:25 AM
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Replies: 111
Views: 83,785
Great discussion and thanks for sharing the...
Great discussion and thanks for sharing the plots. In short, Nextseq generates higher error rate in variant calling. This is often overlooked when buying the machine. It poses great challenges for...
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Forum: General
12-11-2019, 01:46 AM
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Replies: 3
Views: 5,635
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Forum: The Pipeline
12-11-2019, 01:39 AM
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Replies: 1
Views: 12,899
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Forum: General
12-11-2019, 01:32 AM
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Replies: 2
Views: 3,378
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Forum: Bioinformatics
12-10-2019, 05:50 AM
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Replies: 1
Views: 1,190
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Forum: Bioinformatics
12-10-2019, 05:32 AM
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Replies: 2
Views: 1,391
What I will do is to write my own script to
1)...
What I will do is to write my own script to
1) blastn the sequences against itself (hopefully your sequences are long enough to justify using blast)
2) filter the results to remove blastn results...
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Forum: General
07-20-2018, 07:52 AM
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Replies: 1
Views: 1,926
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Forum: General
04-09-2018, 03:13 AM
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Replies: 1
Views: 1,958
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Forum: Bioinformatics
05-09-2017, 06:05 AM
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Replies: 1
Views: 1,440
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Forum: Bioinformatics
08-02-2016, 08:02 AM
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Replies: 2
Views: 1,537
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Forum: Bioinformatics
08-02-2016, 07:55 AM
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Replies: 1
Views: 1,219
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Forum: Bioinformatics
07-15-2016, 05:51 AM
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Replies: 6
Views: 2,564
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Forum: Introductions
10-13-2015, 12:42 PM
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Replies: 1
Views: 977
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Forum: General
05-08-2015, 01:10 PM
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Replies: 5
Views: 2,637
Will be nice to block all posts not written in...
Will be nice to block all posts not written in English. I've been irritated by non-English threads especially job postings despite the fact that google translate works very well nowadays. This kind...
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Forum: Bioinformatics
05-06-2015, 11:37 PM
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Replies: 9
Views: 1,620
Hmm... I'm curious if there is such a list. A...
Hmm... I'm curious if there is such a list. A lowly expressed gene is either not activated or repressed. I think some people are overly concern about not getting sufficient depth of lowly expressed...
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Forum: General
05-05-2015, 05:10 PM
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Replies: 12
Views: 3,265
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Forum: Epigenetics
04-08-2015, 10:50 AM
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Replies: 3
Views: 2,214
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Forum: Epigenetics
02-12-2015, 11:03 AM
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Replies: 3
Views: 2,214
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Forum: Epigenetics
02-04-2015, 02:55 PM
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Replies: 3
Views: 2,214
Why sonicate DNA to 200-800bp?
Hi guys,
I don't understand why Illumina recommends an insert size of 200-800bp for Truseq ChIP library preparation when the protocol selects only 250-300 bp fragments for sequencing. Since...
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Forum: Bioinformatics
01-23-2015, 02:17 PM
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Replies: 1
Views: 1,226
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Forum: Introductions
11-06-2014, 02:34 PM
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Replies: 2
Views: 1,434
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Forum: Academic/Non-Profit Jobs
07-01-2014, 12:46 PM
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Replies: 20
Views: 3,785
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Forum: Academic/Non-Profit Jobs
07-01-2014, 11:47 AM
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Replies: 20
Views: 3,785
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