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Forum: Metagenomics 04-19-2018, 09:42 AM
Replies: 13
Views: 7,672
Posted By thermophile
good to know, thanks

good to know, thanks
Forum: Metagenomics 04-18-2018, 11:28 AM
Replies: 13
Views: 7,672
Posted By thermophile
have you ever tried ampure cleaning after the...

have you ever tried ampure cleaning after the denaturing step? That should work, right? Carboxyl groups would attract either single or double stranded. But maybe [PEG] would need to be changed?
Forum: Metagenomics 04-17-2018, 01:48 PM
Replies: 13
Views: 7,672
Posted By thermophile
interesting. I've had a few library pools with a...

interesting. I've had a few library pools with a drop off after 50ish bases but couldn't see primer diamers on the tape station (I don't routinely run my amplicon libraries on the tape station, just...
Forum: Metagenomics 04-17-2018, 11:53 AM
Replies: 13
Views: 7,672
Posted By thermophile
Phillip-what conditions have you seen primers...

Phillip-what conditions have you seen primers annealed to full length amplicons? do you routinely heat denature then snap cool before cleaning amplicons?

OP- I look at %base to see what is...
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