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Forum: Metagenomics 04-17-2018, 03:23 AM
Replies: 13
Views: 7,673
Posted By AmitChaurasia
Thanks a lot Phillip for your valuable inputs. ...

Thanks a lot Phillip for your valuable inputs.

I have gone through your suggestions and looked into the data in greater details wrt possiblity of primer-dimer or adapter dimers. I have checked the...
Forum: Metagenomics 04-15-2018, 07:48 PM
Replies: 13
Views: 7,673
Posted By AmitChaurasia
Hi Kinman, Thanks for your reply, I have...

Hi Kinman,

Thanks for your reply, I have taken random samples to check with Bioanalyzer and no short peaks were found. Also fastq files were checked to have 301 base length only.

So this can...
Forum: Metagenomics 04-15-2018, 02:27 AM
Replies: 13
Views: 7,673
Posted By AmitChaurasia
I had a Metagenomic 16s rRNA run on MiSeq v3 kit 2 x300 cycles. with good PF >89% but

Hi All,

I had a metagenomic run on MiSeq using v3 kits of 16s rRNA v3-v4 region. I have loaded 8pM pooled library with 10% PhiX spike-in. MiSeq run had 89% PF with 16 M raw reads but with very low...
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