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Forum: SOLiD 03-31-2011, 09:48 AM
Replies: 10
Views: 4,034
Posted By pmiguel
Technically, you could do a single cycle of PCR...

Technically, you could do a single cycle of PCR to "step-out" your adapters to their full length. That can result in no PCR duplication. Personally, I would do at least 3 cycles. And would only go...
Forum: SOLiD 03-31-2011, 08:42 AM
Replies: 10
Views: 4,034
Posted By pmiguel
Yes you are talking about a "bottomed out"...

Yes you are talking about a "bottomed out" library. You start with a certain amount of DNA. For SureSelect, I don't know how much. But rule of thumb: 1 ug of DNA fragmented to average 1 kb size...
Forum: SOLiD 03-31-2011, 05:14 AM
Replies: 10
Views: 4,034
Posted By pmiguel
I do not know. But the read name itself is the...

I do not know. But the read name itself is the bead's co-ordinate. The first field is the panel number. So the 2nd and 3rd are probably planar co-ordinates (Cartesian?). Does anyone know? I vaguely...
Forum: SOLiD 03-30-2011, 01:41 PM
Replies: 10
Views: 4,034
Posted By pmiguel
Hi Thibault, The only circumstances under...

Hi Thibault,
The only circumstances under which going to higher densities of beads would result in high % of PCR duplications would be:
(1) There are some high intensity beads bleeding...
Forum: SOLiD 03-30-2011, 11:44 AM
Replies: 10
Views: 4,034
Posted By pmiguel
Hi Thibault, Could you give us a little...

Hi Thibault,
Could you give us a little more information to go on? What type of library? How many cycles of PCR did you do during library construction. (I don't me ePCR, I mean the number of PCR...
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