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Forum: SOLiD

03-31-2011, 09:02 AM

Replies: 10

PCR duplicates increase when excess of beads

Views: 4,034

Posted By tdm

Hi niceday With SOLID protocol, it is not...

Hi niceday

With SOLID protocol, it is not possible to go into enrichment without PCR because adapters are truncated and we need the nick translation step to attach correctly the adpaters.
thank...

Forum: SOLiD

03-31-2011, 05:19 AM

Replies: 10

PCR duplicates increase when excess of beads

Views: 4,034

Posted By tdm

I should be able to figure something with...

I should be able to figure something with coordinates in color cfasta file.
thank you
thibault

Forum: SOLiD

03-30-2011, 01:56 PM

Replies: 10

PCR duplicates increase when excess of beads

Views: 4,034

Posted By tdm

I was already thinking about the point number 2. ...

I was already thinking about the point number 2.
I will check about distance between the duplicate of same fragment.
Do you know if picard argument OPTICAL_DUPLICATE_PIXEL_DISTANCE work with SOLID...

Forum: SOLiD

03-30-2011, 12:55 PM

Replies: 10

PCR duplicates increase when excess of beads

Views: 4,034

Posted By tdm

Hi PMiguel, I have the data you ask for: Type...

Hi PMiguel,
I have the data you ask for:
Type of library: SureSelect All Human Exon Capture (50Mb)
Sequencing: Fragment (50bp), barcoded samples, 6 samples/slide
How many cycles of PCR: As low as...

Forum: SOLiD

03-30-2011, 11:37 AM

Replies: 10

PCR duplicates increase when excess of beads

Views: 4,034

Posted By tdm

PCR duplicates increase when excess of beads

Hello,
We are experiencing an increasing of PCR duplicates.
Could this be realated to an increasing of the quantity of beads in order to get more reads?
Thibault

Showing results 1 to 5 of 5

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