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Forum: Sample Prep / Library Generation 05-04-2010, 11:07 AM
Replies: 0
Views: 3,080
Posted By polystone
insert size

I am doing RNA-seq using illumina kit. But i am confusing on the insert size. When performing gel seclection after cDNA being ligated with adaptor, the 200 bp size with cDNA and adaptor is seclected...
Forum: Sample Prep / Library Generation 03-30-2010, 12:21 PM
Replies: 4
Views: 3,260
Posted By polystone
It is purified library for subsequent cluster...

It is purified library for subsequent cluster generation in mRNA-seq. This library is generated by PCR enrichment during which the gel-seclection size at 200 bp is used as template. The PCR cycle is...
Forum: Sample Prep / Library Generation 03-29-2010, 11:52 AM
Replies: 4
Views: 3,260
Posted By polystone
bioanalyzer image

I am preparing the library for single end RNA-seq following the illumina protocol. However, by agilent analysis for library, the two smear bands occur at 200bp and 500 bp. I do not know why the 500...
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