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Search: Posts Made By: steinmann
Forum: Ion Torrent 04-18-2013, 07:17 AM
Replies: 70
Views: 20,392
Posted By steinmann
You confuse me do too :)

You confuse me do too :)
Forum: Ion Torrent 04-17-2013, 04:27 AM
Replies: 70
Views: 20,392
Posted By steinmann
Guess my timing was not perfect but I did well...

Guess my timing was not perfect but I did well enough :)
Forum: RNA Sequencing 03-15-2013, 06:04 AM
Replies: 3
Views: 1,437
Posted By steinmann
Start by trying to ask the right questions. Then...

Start by trying to ask the right questions. Then the answer should become obvious.

The answer will depends on what you want to do with these data. qPCR validation may not be needed at all.
Forum: Ion Torrent 03-15-2013, 05:10 AM
Replies: 70
Views: 20,392
Posted By steinmann
Have sold my shares for now. Not sure whether the...

Have sold my shares for now. Not sure whether the steep increase in price is fully justified. Reports over poor quality and shorter read lengths on the proton make me skeptical.
Forum: Ion Torrent 03-14-2013, 08:55 AM
Replies: 9
Views: 5,630
Posted By steinmann
Thanks for lettings us know! Was this attempting...

Thanks for lettings us know! Was this attempting to do 100 or 200 bp sequencing? Sounds disastrous.
Forum: Ion Torrent 02-20-2013, 01:30 AM
Replies: 70
Views: 20,392
Posted By steinmann
Funny that you say that. Intel happens to be my...

Funny that you say that. Intel happens to be my second largest long position :)
Forum: Ion Torrent 02-19-2013, 02:54 AM
Replies: 70
Views: 20,392
Posted By steinmann
Dont think the homopolymer issue is a...

Dont think the homopolymer issue is a showstopper. To me it looks like they have improved to a level that can be considered good enough.

As for the possible competitors you mentioned its hard to...
Forum: Ion Torrent 02-15-2013, 05:38 AM
Replies: 70
Views: 20,392
Posted By steinmann
A more likely outcome is that one method is...

A more likely outcome is that one method is inherently superior and will kill the competitor.
Forum: Ion Torrent 02-14-2013, 02:51 AM
Replies: 70
Views: 20,392
Posted By steinmann
* The proton II chip should be shipping next...

* The proton II chip should be shipping next month.
* An answer to Nextera already exists: http://products.invitrogen.com/ivgn/product/4480829
* Slow pace? You consider 10x improvement every 6...
Forum: Sample Prep / Library Generation 02-13-2013, 04:04 AM
Replies: 3
Views: 1,205
Posted By steinmann
http://www.biomedcentral.com/1471-2164/14/90/abstr...

http://www.biomedcentral.com/1471-2164/14/90/abstract
Forum: Illumina/Solexa 02-11-2013, 03:10 AM
Replies: 11
Views: 3,856
Posted By steinmann
Or start looking like Kodak in 2001 :)

Or start looking like Kodak in 2001 :)
Forum: Illumina/Solexa 02-08-2013, 06:34 AM
Replies: 11
Views: 3,856
Posted By steinmann
In that case one should have expected a little...

In that case one should have expected a little more form Illumina. If things continue to go according to schedule for Ion Torrent the PII chip will be shipping next month.
Forum: Ion Torrent 01-18-2013, 08:30 AM
Replies: 70
Views: 20,392
Posted By steinmann
I did read it very carefully. All I said is that...

I did read it very carefully. All I said is that it looks like someone wants to buy them.

To me all of this is rather odd. Where did the financial post get that information from? From the official...
Forum: Ion Torrent 01-17-2013, 11:55 PM
Replies: 70
Views: 20,392
Posted By steinmann
Looks like someone with serious money agrees with...

Looks like someone with serious money agrees with me and wants to buy the whole company.
...
Forum: Ion Torrent 11-02-2012, 10:47 AM
Replies: 70
Views: 20,392
Posted By steinmann
Well that's some truly exciting news!

Well that's some truly exciting news!
Forum: Ion Torrent 10-26-2012, 09:25 AM
Replies: 88
Views: 148,049
Posted By steinmann
Doing single cell genome sequencing is more about...

Doing single cell genome sequencing is more about the library prep than the sequencer you use. In principle the Proton should do the job just as wells as the HiSeq. The more important question you...
Forum: Ion Torrent 10-25-2012, 03:49 AM
Replies: 70
Views: 20,392
Posted By steinmann
Yes, they mentioned the second (300 million)...

Yes, they mentioned the second (300 million) milestone payment in the Q1 earnings report.
Forum: Ion Torrent 10-19-2012, 07:24 AM
Replies: 70
Views: 20,392
Posted By steinmann
I know you are just trolling, but you raise an...

I know you are just trolling, but you raise an important point. Given what we have experienced with Helicos, PacBio and Oxford Nanopore why should we expect Ion Torrent to be any different?

1.:...
Forum: Ion Torrent 10-19-2012, 03:09 AM
Replies: 70
Views: 20,392
Posted By steinmann
Out of what hat do you pull these numbers? Lets...

Out of what hat do you pull these numbers? Lets set these things straight and do some oversimplified math on the actual numbers.

1.: Life has paid 725 million for Ion Torrent.
2.: Illumina is...
Forum: Ion Torrent 10-17-2012, 04:24 AM
Replies: 70
Views: 20,392
Posted By steinmann
Not going to happen? Its already happening!...

Not going to happen? Its already happening! Plenty of smaller institutes in academia and medical diagnostic companies are now buying sequencers because of the lower initial investment and cost per...
Forum: Ion Torrent 10-16-2012, 08:37 AM
Replies: 70
Views: 20,392
Posted By steinmann
Yes! And a fairly small part as of now. But the...

Yes! And a fairly small part as of now. But the sequencing market is large enough to become a major part of it.



Why would you think so?



That whole part makes little sense to me. You...
Forum: Ion Torrent 09-26-2012, 12:53 AM
Replies: 70
Views: 20,392
Posted By steinmann
Yes, nothing but a claim. But a member of this...

Yes, nothing but a claim. But a member of this forum suggested that he had indeed seen such improvements. Posted yesterday in this thread at 2:40 PM but post got lost. Maybe linked to recent...
Forum: Ion Torrent 09-25-2012, 03:08 AM
Replies: 70
Views: 20,392
Posted By steinmann
Their claim is that the improvements were made...

Their claim is that the improvements were made through changes in chemistry (since introduction of the 300 bp kit) and base calling algorithm (torrent suite 3.0).
Forum: Ion Torrent 09-24-2012, 12:44 AM
Replies: 70
Views: 20,392
Posted By steinmann
This: http://www.iontorrent.com/lomanpaper ...

This:
http://www.iontorrent.com/lomanpaper

And the recent 400 bp run on the 318 chip:
http://ioncommunity.lifetechnologies.com/community/datasets/datasets_by_chip

Indicate that they have been...
Forum: Ion Torrent 09-23-2012, 09:02 AM
Replies: 70
Views: 20,392
Posted By steinmann
The chip has 165 wells. Given a yield of 36-48%...

The chip has 165 wells. Given a yield of 36-48% 60-80 million reads is what you should expect. I would guess that thats quite realistic and the 10 Gb spec is rather conservative.

I agree that its...
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