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Forum: Illumina/Solexa 10-18-2017, 01:37 PM
Replies: 12
Views: 967
Posted By provirus
Hi Austin. Is it correctly understood that you...

Hi Austin. Is it correctly understood that you had the low diversity regions/patches offset from one another by using 2+ different adapter/amplicon ends (effectively 'moving' the patches relatively...
Forum: Illumina/Solexa 10-18-2017, 11:41 AM
Replies: 12
Views: 967
Posted By provirus
Hi Austin, interestingly. I will have a look in...

Hi Austin,
interestingly. I will have a look in the folders, and see if it makes any sense. You are right that PhiX spike would help. We are testing this at 35-45% at the expense of...
Forum: Illumina/Solexa 10-18-2017, 10:12 AM
Replies: 12
Views: 967
Posted By provirus
Thanks. We will indeed approach Illumina with...

Thanks. We will indeed approach Illumina with this. I wanted to know if some users had experience with this, and also to learn about the "inner workings" of the programs that control the runs - it is...
Forum: Illumina/Solexa 10-17-2017, 01:44 PM
Replies: 12
Views: 967
Posted By provirus
Making a custom run recipe on nextseq 500

Is it possible to design a complete custom run recipe by yourself on a Nextseq500. For instance I am considering a PE sequence run were some specific cycles are chemistry-only (with no pictures...
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