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Forum: Bioinformatics 11-13-2018, 10:11 PM
Replies: 5
Views: 1,535
Posted By ARDISSON
Hi, I mapped separately paired end and single...

Hi,
I mapped separately paired end and single end reads. Then I sorted the BAMs with the picard tools with the option CREATE_INDEX=FALSE and it goes well. Then I tried to merge them, but with the...
Forum: Bioinformatics 11-12-2018, 01:28 AM
Replies: 5
Views: 1,535
Posted By ARDISSON
Hello, Thank you for your answer. But I have a...

Hello,
Thank you for your answer. But I have a new problem.
When I try to create the BAM index, i encounter a new problem. The BAM index format seems to be not fit for Reference sequence above...
Forum: Bioinformatics 11-06-2018, 11:24 PM
Replies: 5
Views: 1,535
Posted By ARDISSON
reference size for BWA index

Dear all,
I would like to take a mapping with bwa_mem on a genomic reference of 10GB but I have a problem with bwa index.
Is there a maximum size for the reference with bwa index?
how to make the...
Forum: Pacific Biosciences 08-02-2016, 11:02 PM
Replies: 4
Views: 1,887
Posted By ARDISSON
I'll start paying attention to these 3 points. ...

I'll start paying attention to these 3 points.
Thanks
Forum: Pacific Biosciences 08-02-2016, 04:30 AM
Replies: 4
Views: 1,887
Posted By ARDISSON
Hi, the problem is not for instance low yield...

Hi,
the problem is not for instance low yield or wrong size, I think is a incompatibility between reaction product PCR and SPRI AMPure XP beads, because during water elution, beads make a strange...
Forum: Pacific Biosciences 08-01-2016, 05:42 AM
Replies: 4
Views: 1,887
Posted By ARDISSON
Long range PCR / purication AMpure XP

Hi,
I make a librairies around 6Kb for sequencing.
I shear and size DNA and I add adaptator by ligation. After each step, I do a purification with 1x SPRI AMPure XP beads without particular...
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