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Forum: RNA Sequencing 08-28-2014, 08:53 PM
Replies: 15
Views: 22,241
Posted By Jeremy
For differential expression analysis, I wouldn't....

For differential expression analysis, I wouldn't. That design would have a lot of trouble getting published. For almost the same price you can sequence biological replicates that have been...
Forum: RNA Sequencing 08-28-2014, 08:28 PM
Replies: 15
Views: 22,241
Posted By Jeremy
Based on my quote marks I think I was asking the...

Based on my quote marks I think I was asking the OP what they meant and then pointing out (via rhetorical question) that you can't get within group variance using a pooled approach. But it was so...
Forum: RNA Sequencing 11-24-2010, 07:26 PM
Replies: 15
Views: 22,241
Posted By Jeremy
By "mix multiple biological replicates in the...

By "mix multiple biological replicates in the same barcoded sample", do you mean each sample has its own barcode and is pooled together and sequenced. Or do you mean that multiple samples are pooled...
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