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Forum: Sample Prep / Library Generation 11-04-2016, 07:41 AM
Replies: 18
Views: 13,008
Posted By DStephens@NuGEN
Hi MatthewHaas, The Qiagen RNAeasy kits...

Hi MatthewHaas,

The Qiagen RNAeasy kits include separate protocols for RNA Clean-up after DNase treatment. All you need to do is bring your sample volume up to 100 uL with nuclease-free water, add...
Forum: Illumina/Solexa 07-19-2016, 08:02 AM
Replies: 1
Views: 1,332
Posted By DStephens@NuGEN
Hi rgejman, Our Ovation SoLo RNA-Seq System...

Hi rgejman,
Our Ovation SoLo RNA-Seq System uses a custom primer to overcome low-diversity sequence at the 5 end of the library. Like your custom primer, there is overlap with the SP1 sequence and...
Forum: RNA Sequencing 07-05-2016, 04:48 PM
Replies: 5
Views: 2,519
Posted By DStephens@NuGEN
Hi sbchua.1990, We have seen traces such as...

Hi sbchua.1990,
We have seen traces such as these when the chip priming/loading was not performed correctly. Did you ensure that the chip priming station plunger was set to the highest notch and the...
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