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Search: Posts Made By: Allison Harmon
Forum: Metagenomics 08-15-2017, 10:50 AM
Replies: 0
Views: 2,690
Posted By Allison Harmon
recA database?

I will like to do a metagenomics experiment using the recA gene as the target. 16s does not provide enough resolution for the bacteria I am interested in (BCC complex). Are there any publicaly...
Forum: Metagenomics 11-22-2014, 01:29 PM
Replies: 4
Views: 1,519
Posted By Allison Harmon
I am looking to amplify regions v3 and v4...

I am looking to amplify regions v3 and v4 following the Illumina protocol.
It is about 460bp
Forum: Metagenomics 11-22-2014, 01:10 PM
Replies: 4
Views: 1,519
Posted By Allison Harmon
MiSeq v2 cartridges for metagenomics

Hi all,
Has anyone used the MiSeq v2 cartridges for 16s metagenomics? Illumina recommends using the v3 cartridges for better metrics. I have plenty of v2 on hand that will like to use for...
Forum: Illumina/Solexa 09-21-2014, 05:05 PM
Replies: 55
Views: 30,461
Posted By Allison Harmon
No. The protocol you refer to targets regions V3...

No. The protocol you refer to targets regions V3 and V4 using 2 x 300 cartridges. I want to yarget region v4 using 2 x 250 cartridges. Illumina has an application note on this subject, but it offers...
Forum: Illumina/Solexa 09-13-2014, 02:08 PM
Replies: 55
Views: 30,461
Posted By Allison Harmon
MiSeq for 16s sequencing of V4

Hello! I will like to do metagenomic sequencing of the V4 region. I will like to use 2 x 250 cartridges, Nextera XT kits and Illumina manufactured indexes. Is there a detailed protocol for doing...
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