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Forum: Sample Prep / Library Generation 12-22-2017, 01:14 PM
Replies: 4
Views: 3,092
Posted By MasterSeq
why VN, why not just V for oligo dT anchor?

Why does almost everyone use 3'-VN as the anchor for oligo dT primers rather than using 3'-V?

The former only has a 1/12 chance of matching a given mRNA, vs. 1/3 for the latter. Plus 1/4 of the...
Forum: Illumina/Solexa 12-20-2015, 06:36 AM
Replies: 9
Views: 10,463
Posted By MasterSeq
Unhappy What does "topping off" mean?

Ok, I know what it means, but how does one do it? Collect leftover reagent from used reagent cartridges? "Borrow" from a new cartridge?

My run stopped after clustering. Message was "No usable...
Forum: Epigenetics 03-04-2015, 09:41 AM
Replies: 26
Views: 10,779
Posted By MasterSeq
I know this is an old thread but this could be...

I know this is an old thread but this could be useful for somebody: NuGEN just launched a kit specifically for RRBS which should be much better than using their Methyl-Seq kit. It uses direct...
Forum: Sample Prep / Library Generation 03-04-2015, 09:37 AM
Replies: 9
Views: 4,434
Posted By MasterSeq
Read 1 (Forward read) is in the sense orientation...

Read 1 (Forward read) is in the sense orientation for NuGEN stranded libraries.
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