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Forum: General 03-03-2010, 10:35 AM
Replies: 8
Views: 2,974
Posted By cgb
seems oddly unpopular with the French ?

seems oddly unpopular with the French ?
Forum: Illumina/Solexa 03-01-2010, 10:37 AM
Replies: 8
Views: 3,897
Posted By cgb
Almost. In fact it is quite hard to make huge...

Almost. In fact it is quite hard to make huge improvements as Nava showed in SWIFT. The whole platform has been revised in parallel to the pipeline. This includes changes to optics, affecting pixels...
Forum: Illumina/Solexa 01-26-2010, 11:20 PM
Replies: 74
Views: 363,710
Posted By cgb
the chemistry is proprietary.

the chemistry is proprietary.
Forum: General 12-21-2009, 04:46 AM
Replies: 67
Views: 79,005
Posted By cgb
Love the map

But by my calculation it is only catching about 15% of all the installations. How do we get the rest to sign up ?
Forum: General 12-05-2009, 08:51 AM
Replies: 35
Views: 12,868
Posted By cgb
discounts

of course what we can never expect to see is how many machines are fully paid for, how many are discounted and how many are just placed for free.
Forum: General 11-21-2009, 05:39 AM
Replies: 8
Views: 7,562
Posted By cgb
claims

I saw a post on another site saying 1500 Solexa systems out.

I would guess that gives it ~70% market, maybe 20% for 454 and 10 for SOLiD ?
Forum: General 11-15-2009, 06:41 AM
Replies: 35
Views: 12,868
Posted By cgb
whilst I also don't have access to any official...

whilst I also don't have access to any official numbers either, your estimate sounds about right -- based on what ive heard.
Forum: General 11-08-2009, 12:32 PM
Replies: 8
Views: 7,562
Posted By cgb
Here is my view on the Illumina(Solexa) dominance...

Here is my view on the Illumina(Solexa) dominance as things stand (and could easily change ! - this is a very dynamic space)

1. The overall 'workflow' for the sequencing process on this platform...
Forum: General 11-08-2009, 12:24 PM
Replies: 8
Views: 7,562
Posted By cgb
Lynx

Solexa ltd existed as a UK company before it reversed into Lynx Therapeutics to form Solexa Inc in 2004. MPSS, the Lynx technology (in many ways pioneering short read sequencing and technically...
Forum: Epigenetics 08-31-2009, 01:58 AM
Replies: 4
Views: 8,143
Posted By cgb
nanopores !

nanopores !
Forum: 454 Pyrosequencing 08-19-2009, 09:17 PM
Replies: 20
Views: 8,274
Posted By cgb
might be your library prep. see the Turner et al...

might be your library prep. see the Turner et al paper.
Forum: Academic/Non-Profit Jobs 06-26-2009, 05:08 AM
Replies: 7
Views: 3,186
Posted By cgb
cambridge

where you live, is europe's largest bio-hub.
Forum: General 05-31-2009, 08:45 AM
Replies: 5
Views: 6,446
Posted By cgb
i agree with biowizard. smaller search space does...

i agree with biowizard. smaller search space does not guarantee higher accuracy/sensitivity.
Forum: Illumina/Solexa 05-05-2009, 09:22 PM
Replies: 6
Views: 2,494
Posted By cgb
James, I always thought the GAs looked like...

James,

I always thought the GAs looked like Hewey, Dewey and Louie (film :Silent Running 1971 - not the ducks) ?
Forum: General 12-31-2008, 09:08 AM
Replies: 3
Views: 4,409
Posted By cgb
Ive never understood where the numbers in point 1...

Ive never understood where the numbers in point 1 come from. In total the cost of computing to run something like a GAII is now about $10K per instrument (it used to be more like $40K). That's around...
Forum: Illumina/Solexa 12-21-2008, 11:05 PM
Replies: 1
Views: 1,948
Posted By cgb
sounds like 'blobs' - have a look at...

sounds like 'blobs' - have a look at www.genographia.org
Forum: Bioinformatics 12-21-2008, 11:03 PM
Replies: 3
Views: 2,267
Posted By cgb
this looks like it in the ipar code, which you...

this looks like it in the ipar code, which you dont need. is there a compile option for --no-ipar ? otherwise modify the makefiles.
Forum: Bioinformatics 12-14-2008, 01:52 AM
Replies: 4
Views: 2,737
Posted By cgb
some of your sequences are artifacts of poor...

some of your sequences are artifacts of poor image quality, flowcell imperfections etc. in which case you need to use X,Y co-ordinates as part of your filtering - and/or cross check the images.
Forum: Bioinformatics 11-20-2008, 10:46 PM
Replies: 236
Views: 566,185
Posted By cgb
a few brief comments on this post : the...

a few brief comments on this post :

the short read aligners like MaQ and eland dont 'throw reads one at a time" - in fact they do very efficient batch based inexact matching and some fancy maths...
Forum: Bioinformatics 11-17-2008, 11:54 PM
Replies: 15
Views: 6,828
Posted By cgb
works for me

works for me
Forum: Illumina/Solexa 11-13-2008, 05:20 AM
Replies: 10
Views: 11,234
Posted By cgb
comparison

Don't be bullied - you are the customer ! Ive heard so much fooey over the years its a bit rich being accused of spreading false information by a vendor!

1. there are no accurate costings out...
Forum: Illumina/Solexa 10-15-2008, 08:26 AM
Replies: 1
Views: 1,661
Posted By cgb
put some data up on www.genographia.org so that...

put some data up on www.genographia.org so that we can see it. there are still imaging issues on the GA - they can be fixed once identified.
Forum: Illumina/Solexa 10-14-2008, 03:51 PM
Replies: 5
Views: 4,368
Posted By cgb
yes there was a solexa video on the website for a...

yes there was a solexa video on the website for a long time - i sure it was handed out at conferences...
Forum: The Pipeline 10-06-2008, 11:50 AM
Replies: 9
Views: 5,829
Posted By cgb
they win the prize for promises.

they win the prize for promises.
Forum: Illumina/Solexa 10-01-2008, 09:36 PM
Replies: 8
Views: 3,413
Posted By cgb
that doesn't look right.

that doesn't look right.
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