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Forum: Academic/Non-Profit Jobs 07-10-2019, 07:04 AM
Replies: 0
Views: 235
Posted By scotto
NGS laboratory position at St. Jude - Memphis

I have an open position in an NGS production core facility. Please contact me for additional information or check out the posting:
...
Forum: Academic/Non-Profit Jobs 03-05-2019, 06:10 AM
Replies: 0
Views: 335
Posted By scotto
NGS Laboratory Position at St. Jude

I have an open position in a well resourced NGS production core facility. Duties include input QC, library prep, library QC, and instrument operation. Strong molecular biology skills are required....
Forum: Academic/Non-Profit Jobs 08-07-2018, 11:58 AM
Replies: 0
Views: 541
Posted By scotto
NGS Laboratory Position at St. Jude

We are recruiting for a laboratory position in the NGS core facility.

https://careers-stjude.icims.com/jobs/3916/genome-sequencing-analyst-ii/job
Forum: Illumina/Solexa 03-29-2016, 01:25 PM
Replies: 12
Views: 2,446
Posted By scotto
Since we first noticed this in mid-January, all...

Since we first noticed this in mid-January, all of our paired end v3 runs (~35) have been affected to some extent, but the extent of the gradient varies from almost normal looking to horrible. The...
Forum: Illumina/Solexa 03-29-2016, 07:03 AM
Replies: 12
Views: 2,446
Posted By scotto
We have been seeing this for months. Tech support...

We have been seeing this for months. Tech support says it is v3 SBS reagents, but they can't narrow it to specific lot numbers.
Forum: Academic/Non-Profit Jobs 06-08-2014, 06:21 AM
Replies: 0
Views: 3,896
Posted By scotto
Laboratory position at St. Jude Children's Research Hospital

Job Title Genome Sequencing Analyst II
Department Hartwell Center
Job Number 32343

Job Description:
The Genome Sequencing Analyst II assists in the operation of the laboratory by...
Forum: Illumina/Solexa 08-07-2013, 08:15 AM
Replies: 55
Views: 30,438
Posted By scotto
We used it for microbial metagenomics starting...

We used it for microbial metagenomics starting with 50 ng of DNA from a fecal extract. We didn't use any human input. We spiked in about 5-10% phiX to help the basecaller overcome the low diversity.
Forum: Illumina/Solexa 07-02-2013, 07:11 AM
Replies: 55
Views: 30,438
Posted By scotto
We have successfully run v4 16s samples on the...

We have successfully run v4 16s samples on the MiSeq using the Caporaso method and the Bioo Scientific product, which is just a kitted version of the Caporaso method. Both have worked with MiSeq...
Forum: Academic/Non-Profit Jobs 05-30-2012, 10:06 AM
Replies: 0
Views: 879
Posted By scotto
Genome Sequencing Analyst at St. Jude Children's Research Hospital

The Genome Sequencing core at the Hartwell Center for Bioinformatics and Biotechnology provides state of the art genome sequencing analysis to investigators at St. Jude Children’s Research Hospital...
Forum: Academic/Non-Profit Jobs 11-10-2011, 10:30 AM
Replies: 0
Views: 1,444
Posted By scotto
Genome Sequencing Scientist at St. Jude Children's Research Hospital

The Genome Sequencing core at the Hartwell Center for Bioinformatics and Biotechnology provides state of the art genome sequencing analysis to investigators at St. Jude Children’s Research Hospital...
Forum: Academic/Non-Profit Jobs 03-08-2011, 01:06 PM
Replies: 0
Views: 1,643
Posted By scotto
DNA sequencing and Genotyping Scientist at St. Jude Children’s Research Hospital

The Genome Sequencing core at the Hartwell Center for Bioinformatics and Biotechnology provides state of the art genome sequencing analysis to investigators at St. Jude Children’s Research Hospital...
Forum: Academic/Non-Profit Jobs 09-23-2010, 08:30 AM
Replies: 0
Views: 1,609
Posted By scotto
DNA sequencing and Genotyping Scientist at St. Jude Children’s Research Hospital

The Genome Sequencing core at the Hartwell Center for Bioinformatics and Biotechnology provides state of the art genome sequencing analysis to investigators at St. Jude Children’s Research Hospital...
Forum: 454 Pyrosequencing 08-13-2010, 09:22 AM
Replies: 42
Views: 12,792
Posted By scotto
Using the shotgun analysis instead of amplicon...

Using the shotgun analysis instead of amplicon got most of the parameters in spec. The raw reads are still low, but that's another issue.
Forum: 454 Pyrosequencing 08-03-2010, 07:01 AM
Replies: 42
Views: 12,792
Posted By scotto
Thanks, Phillip. We submitted our troubleshooting...

Thanks, Phillip. We submitted our troubleshooting form and gsSupportTool output on Thursday and have only gotten an automated email confirming receipt. I don't know how long it usually takes to get a...
Forum: 454 Pyrosequencing 07-29-2010, 05:50 AM
Replies: 42
Views: 12,792
Posted By scotto
We were having problems with a customer's...

We were having problems with a customer's amplicon library. Roche sent us a control amplicon library to run side by side. The Roche library had the highest percentage of short quality failures,...
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