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Forum: Bioinformatics 06-10-2019, 05:20 AM
Replies: 2
Views: 812
Posted By frascom
It Should not take that long to FTP into Galaxy. ...

It Should not take that long to FTP into Galaxy.

Try the Galaxy server https://usegalaxy.eu/ I think its great and good resources about how to upload data and analyse. I'm not sure if it has...
Forum: Sample Prep / Library Generation 06-07-2019, 07:07 AM
Replies: 0
Views: 614
Posted By frascom
NEB Fragmentase mitogenome advice

Hi, I am currently working with mitogenome sequencing of small mammals. I have amplified 5 PCR amplicons of the mitogenome around 4-5kb fragment lengths. My next step is to pool fragments and...
Forum: Sample Prep / Library Generation 05-23-2019, 05:02 AM
Replies: 4
Views: 807
Posted By frascom
I have amplified the mitogenome separately so...

I have amplified the mitogenome separately so there should no concern of reads. The main aim is trying to save some costs due to barcoding 192 individuals whilst achieving a high quality of reads. ...
Forum: Sample Prep / Library Generation 05-22-2019, 09:54 AM
Replies: 4
Views: 807
Posted By frascom
TrueSeq PCR free vs Nextera Mitogenome seq

I wondered if anyone had any recommendations for TrueSeq PCR free kits vs Nextera XT for mitogenome sequencing around 17,000bp pooling PCR fragments and around 192 individual samples on a single...
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