Forum: Bioinformatics
Today, 02:22 PM
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Replies: 8
Views: 639
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Forum: Bioinformatics
05-24-2012, 05:52 PM
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Replies: 1
Views: 329
quality management
Hi All,
I want to ask if I do different trimming for different datasets (some kept 50bp, some are with 80bp left). does it influence DE analysis? :confused:
Christina
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Forum: Bioinformatics
06-28-2011, 10:07 PM
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Replies: 170
Views: 27,671
problem with SSPACE
Hi
I have a problem of using SSPACE. the process was failed at bowtie-build. I can use bowtie to do index with my genome file, but it seems not working in SSPACE... What's the problem?
Thanks
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Forum: Bioinformatics
06-28-2011, 07:25 PM
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Replies: 16
Views: 2,518
bowtie-build error
Hi boetsie,
SSPACE must be very useful tool for scaffolding. But when I tried to use it, the process was failed by bowtie-build step. I only have contig file contains all name with super_contig...
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Forum: RNA Sequencing
05-01-2011, 10:48 PM
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Replies: 18
Views: 2,994
Hi Simon,
Can you post out the literatures...
Hi Simon,
Can you post out the literatures related to comparing GE between using microarray and RNA-Seq, and prove your views? Even though I agree with you, but I'd like to see some published...
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Forum: RNA Sequencing
05-01-2011, 10:06 PM
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Replies: 18
Views: 2,994
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Forum: Illumina/Solexa
05-01-2011, 08:51 PM
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Replies: 4
Views: 918
Hi suludana,
Thank you!
Are there any...
Hi suludana,
Thank you!
Are there any literatures described how large is the expected read output (Gbases) for each sample in illumina RNA-Seq? (mammalian) if the genome is 3Gbases.
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Forum: Illumina/Solexa
04-27-2011, 11:54 PM
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Replies: 4
Views: 918
questions about choosing read coverage
Hi everyone,
It sounds stupid question... but I really want to know how you guys choose the read coverage before sequencing. I know it is related to the cost. But What is the minimum size and what...
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