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Search: Posts Made By: lvogel
Forum: Bioinformatics 09-12-2017, 10:37 AM
Replies: 7
Views: 836
Posted By lvogel
OK, thanks. I merge the paired-ends as a first...

OK, thanks. I merge the paired-ends as a first step, yes, if that's what you mean.
Forum: Bioinformatics 09-12-2017, 06:54 AM
Replies: 7
Views: 836
Posted By lvogel
Our primers aren't species-specific. We use the...

Our primers aren't species-specific. We use the same 3 primer sets for all of the mixed samples of insects. We don't know what the species are likely to be until BLASTing at the end.
Forum: Bioinformatics 09-12-2017, 06:38 AM
Replies: 7
Views: 836
Posted By lvogel
Dear GenoMax (or anyone), Is there a reason why...

Dear GenoMax (or anyone),
Is there a reason why assembly should be done at the step where you said (after PE merging and removal of primers/tags), as opposed to waiting until the end, when we have...
Forum: Bioinformatics 09-04-2017, 11:57 PM
Replies: 7
Views: 836
Posted By lvogel
Thumbs up Thanks

Thanks for the reply. Sounds good. I'll give it a try, and post again if I have problems.
Forum: Bioinformatics 09-04-2017, 06:34 AM
Replies: 7
Views: 836
Posted By lvogel
Question Assembling mini barcodes into full-length gene

Hi,
I have metabarcoding reads from an Illumina MiSeq run. I usually use a primer set which amplifies a certain fragment of COI. Now, however, we have used three different primer sets which produce...
Forum: General 05-18-2017, 03:36 AM
Replies: 2
Views: 1,092
Posted By lvogel
I have found a (partial) answer to my own...

I have found a (partial) answer to my own question, by reading a paper involving barcoding of tea samples, which I'm also interesting in doing. It seems that it was enough to confirm the presence of...
Forum: General 04-25-2017, 05:45 AM
Replies: 2
Views: 1,092
Posted By lvogel
Question Plant DNA barcoding markers

Hi, I have a general question about DNA barcoding of plants (I just need a brief answer, not a lot of detail). I've heard/read that a combination of matK and rbcL sequences are customarily used to...
Forum: General 11-23-2013, 04:22 AM
Replies: 3
Views: 4,829
Posted By lvogel
Thumbs up Re: hash table

Thank you so much for taking the time to answer. Actually, I think you're probably right. The supervisor I'm doing research with expects me to mostly figure things out for myself. I asked her about...
Forum: General 11-22-2013, 06:34 AM
Replies: 3
Views: 4,829
Posted By lvogel
Question Hash Table

Hi all,
I am a total beginner with bioinformatics, and I am supposed to build a hash table. I have been reading up on everything (stampy, shell scripts, etc.), but nowhere can I find simple...
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