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Forum: Illumina/Solexa 03-21-2015, 10:14 AM
Replies: 14
Views: 14,578
Posted By ps79
Thanks for the replies, nucacidhunter. I am...

Thanks for the replies, nucacidhunter. I am aware that these products won't cluster, and there are indeed ways to work around the unreliable quantification with respect to optimizing clustering of...
Forum: Illumina/Solexa 03-20-2015, 08:23 AM
Replies: 14
Views: 14,578
Posted By ps79
Sure. As I am using custom primers, I wanted to...

Sure. As I am using custom primers, I wanted to make sure I wasn't getting undesired products, so I set up control PCR reactions with only the N5xx oligo or only the N7xx oligo on some of my...
Forum: Illumina/Solexa 03-19-2015, 06:53 AM
Replies: 14
Views: 14,578
Posted By ps79
On the subject of suppression PCR in Nextera...

On the subject of suppression PCR in Nextera library preps, does anyone know if there is anything special in the Illumina primer cocktails or in the PCR mix that encourages this? Iím seeing evidence...
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