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Forum: Sample Prep / Library Generation

03-01-2016, 06:38 AM

Replies: 11

16S library issues

Views: 5,056

Posted By anatgonca

Hi gsshankar Unfortunately we didn't find a...

Hi gsshankar

Unfortunately we didn't find a solution to our problem. After cutting the band and purify the concentration was too little even after pcr amplification and we could not cluster...

Forum: Sample Prep / Library Generation

06-02-2015, 07:09 AM

Replies: 11

16S library issues

Views: 5,056

Posted By anatgonca

Thanks! The problem about selecting different...

Thanks! The problem about selecting different primers is that the libraries are prepared with a kit...which includes the primers with the illumina adapters etc so changing these would change kit...

Forum: Sample Prep / Library Generation

05-29-2015, 02:58 PM

Replies: 11

16S library issues

Views: 5,056

Posted By anatgonca

Thank you all for your replies. I checked before...

Thank you all for your replies. I checked before how it should look the amplicon from NEXTflex v4 kit and for that reason I contacted your tech help. The answer I got was that it was weird. That's...

Forum: Sample Prep / Library Generation

05-17-2015, 10:08 AM

Replies: 11

16S library issues

Views: 5,056

Posted By anatgonca

16S library issues

Hello to all of you.
I am a newbie in the genomics field, so I address here my problem hoping for some feedback and solving ideas.
I am developing a project for 16S V4 region sequencing to...

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