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Forum: Sample Prep / Library Generation 10-08-2019, 10:58 AM
Replies: 3
Views: 559
Posted By chilipep
Thanks for all the advice, I really appreciate...

Thanks for all the advice, I really appreciate it.

The reason I'm getting total cDNA from single cells (primary B cells in this case) is for the reason you mentioned: banking the cDNA for...
Forum: Sample Prep / Library Generation 10-07-2019, 03:34 PM
Replies: 3
Views: 559
Posted By chilipep
n00b Smart-seq2 questions

Hi there, I'm new to Smart-seq and have a couple questions:

1. Can the dT(30)VN primer be substituted with dT(20)VN, dT(23)VN, etc.?
2. How does Superscript IV compare to SSII or SSIII?
3. Is...
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