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Search: Posts Made By: BioHak
Forum: Ion Torrent 09-27-2019, 11:15 AM
Replies: 2
Views: 1,724
Posted By BioHak
Hello missphelps, Hope you didn't waste a chip...

Hello missphelps,
Hope you didn't waste a chip on that run. The signal differential between all of your nucleotides and the wash buffer should be close to 1000 or greater for any cartridge on the...
Forum: Ion Torrent 08-05-2017, 08:11 AM
Replies: 18
Views: 10,813
Posted By BioHak
In my experience you can save the chip at 4C over...

In my experience you can save the chip at 4C over the weekend and room temp overnight and not see significant difference in results
Forum: Ion Torrent 12-23-2014, 04:09 AM
Replies: 2
Views: 5,569
Posted By BioHak
According to sales reps, you must buy if you want...

According to sales reps, you must buy if you want longer - 400bp reads
Forum: Bioinformatics 04-03-2012, 08:41 AM
Replies: 4
Views: 1,788
Posted By BioHak
slop-tigs, nice.. actually amplicons where yes,...

slop-tigs, nice.. actually amplicons where yes, the 2X read-length is greater than the amplicon length.
Thanks, I'll look for FLASH
Forum: Bioinformatics 04-03-2012, 07:30 AM
Replies: 4
Views: 1,788
Posted By BioHak
Software for splicing paired-end fastq files?

Hello, I am looking for an application that will map paired-end reads and form a consensus sequence. I believe pandaseq can do this, but would appreciate if anyone has another recommendation. ...
Forum: Illumina/Solexa 11-05-2009, 04:48 AM
Replies: 5
Views: 4,530
Posted By BioHak
File Not Found!

Anyone have a copy of this video saved that they could share?
Forum: General 09-15-2009, 10:57 AM
Replies: 3
Views: 1,858
Posted By BioHak
Region-Specific Extraction

I worked with this group a while ago, and the technology was quite promising.

narrated ppt from European Federation of Immunohistocompatibility: ...
Forum: Illumina/Solexa 07-07-2009, 04:14 AM
Replies: 2
Views: 1,524
Posted By BioHak
The files have not been replaced is what I have...

The files have not been replaced is what I have learned. The "p" signifies that the processing was done on the pipeline server and as you have probably noticed, the output is different. illumina TS...
Forum: Illumina/Solexa 05-15-2009, 06:26 AM
Replies: 7
Views: 4,293
Posted By BioHak
We've been using the DNA1000 chips to quant our...

We've been using the DNA1000 chips to quant our samples, has anyone here used the new High-sensitivity chips available from Agilent?
Forum: Illumina/Solexa 04-22-2009, 01:10 PM
Replies: 0
Views: 2,176
Posted By BioHak
Buffer composition in cluster kit v2

Hello,
I've googled away and asked tech support but have not been able to find an answer... Does anyone know the composition of HT1 and HP4 ?
Forum: Illumina/Solexa 03-06-2009, 10:11 AM
Replies: 19
Views: 9,968
Posted By BioHak
If you believe it is an amp by-product, why not...

If you believe it is an amp by-product, why not sequence a sample of it on a capillary instrument and verify?

If your goal is to remove the smaller fragment, we cleanup our samples with the...
Forum: Introductions 12-24-2008, 05:47 AM
Replies: 2
Views: 1,535
Posted By BioHak
We're using the FLX in drug discovery. A pool of...

We're using the FLX in drug discovery. A pool of compounds are covalently linked to a DNA tag which describes the "recipe" for making the compound. The pool of DNA is recovered after several...
Forum: Introductions 12-19-2008, 09:52 AM
Replies: 2
Views: 1,535
Posted By BioHak
Hello from PharmLand -- Philadelphia

Hi,
I'm quite happy to have stumbled upon this resource, my group has had 3 FLX sequencers running for about 18months and they are now my responsibility. Hopefully I'll be able to give back to...
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