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Forum: Illumina/Solexa 08-05-2014, 05:42 PM
Replies: 2
Views: 1,580
Posted By chipgal
127bp adaptor dimer

Hi all,

I ran Bioanalyser on the PCR products of my RNA-Seq library prep, and there are adaptor dimers contamination in all my samples. Some are more serious (sample 1) than the others (sample 3)...
Forum: Sample Prep / Library Generation 05-30-2014, 04:47 PM
Replies: 4
Views: 3,230
Posted By chipgal
degraded clinical RNA samples - to seq or not

Hi,

We have received some human clinical RNA samples. We intend to seq them (Illumina HiSeq) to look for changes in gene expression (protein-coding genes as well as non-coding RNA). We checked the...
Forum: Illumina/Solexa 06-27-2012, 01:42 AM
Replies: 1
Views: 1,808
Posted By chipgal
Sequencing mouse and homan mixed sample in one lane (or one barcode) by RNA-Seq?

Hi,
I am currently doing this mouse cells and human cells co-culture and I am looking at the mutual influence of these 2 types of cells on each other. I want to do RNA-Seq on both the mouse and...
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