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Forum: Bioinformatics 04-01-2019, 11:34 AM
Replies: 7
Views: 974
Posted By sformel
Ah ok, that makes sense. I didn't think about...

Ah ok, that makes sense. I didn't think about those kind of biases. Thanks for your help!
Forum: Bioinformatics 04-01-2019, 11:12 AM
Replies: 7
Views: 974
Posted By sformel
Ok, I understand what you're saying in regards to...

Ok, I understand what you're saying in regards to RNA-seq type libraries.

However, I'm working with a 2-step PCR in which the first step is an amplification of a marker gene, followed by an...
Forum: Bioinformatics 04-01-2019, 10:04 AM
Replies: 7
Views: 974
Posted By sformel
Thanks for the quick reply! But I'm still not...

Thanks for the quick reply! But I'm still not sure I understand.

My thought is that the because the primer matched the DNA fragment during PCR, it can be considered a legitimate copy of a...
Forum: Bioinformatics 04-01-2019, 09:45 AM
Replies: 7
Views: 974
Posted By sformel
should primers be considered biological sequence?

Hi,

Forgive me if this is naive, but I'm still newish to thinking about sequencing. I've begun working with the DADA2 software and the tutorial ...
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