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Forum: Sample Prep / Library Generation 11-06-2015, 05:11 PM
Replies: 9
Views: 2,236
Posted By nucacidhunter
From those 10-15% only a small subset would be in...

From those 10-15% only a small subset would be in size-selection window and end up in ddRAD library. In my experience they are below 3% although it would vary from genome to genome and the enzymes...
Forum: Sample Prep / Library Generation 11-06-2015, 09:39 AM
Replies: 9
Views: 2,236
Posted By nucacidhunter
That theory is incorrect because order of bases...

That theory is incorrect because order of bases in a genome is not random. Two genome with similar size and GC% can have different number of RAD fragments.

In ddRAD 1st cycle of PCR is primed...
Forum: Sample Prep / Library Generation 11-05-2015, 03:27 PM
Replies: 9
Views: 2,236
Posted By nucacidhunter
It seems that it is cutting. If you are doing...

It seems that it is cutting. If you are doing ddRAD, you could ligate adapters and do a PCR. Amplification will be a good indication of how it has worked.
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